Purified anti-mouse CD45 Antibody

Pricing & Availability
30-F11 (See other available formats)
Regulatory Status
Other Names
T200, Ly-5, LCA
Rat IgG2b, κ
Ave. Rating
Submit a Review
Product Citations
BALB/c splenocytes stained with 30-F11 purified, followed by anti-rat IgG FITC
  • 30-F11_Pure_071608.jpg
    BALB/c splenocytes stained with 30-F11 purified, followed by anti-rat IgG FITC
  • 30-F11_Pure_CD45_Antibody_2_102518
    Fresh, frozen mouse spleen was stained with purified CD45 clone 30-F11 conjugated and detected with a FITC CODEX™ oligonucleotide duplex (red). Samples were counterstained with CollagenIV Cy5 (green). Data generated at Akoya Biosciences, Inc. using the CODEX™ technology.
Cat # Size Price Quantity Check Availability Save
103101 50 µg £21.00
Check Availability

Need larger quantities of this item?
Request Bulk Quote
103102 500 µg £65.00
Check Availability

Need larger quantities of this item?
Request Bulk Quote

CD45 is a 180-240 kD glycoprotein also known as the leukocyte common antigen (LCA), T200, or Ly-5. It is a member of the protein tyrosine phosphatase (PTP) family, expressed on all hematopoietic cells except mature erythrocytes and platelets. There are different isoforms of CD45 that arise from variable splicing of exons 4, 5, and 6, which encode A, B, and C determinants, respectively. CD45 plays a key role in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation state of the cell as well as cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.

Product Details
Technical Data Sheet (pdf)

Product Details

Antibody Type
Host Species
Mouse thymus or spleen
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
The antibody was purified by affinity chromatography.
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.

FC - Quality tested
IHC-F, CyTOF® - Verified
IP, CMCD, IHC, WB - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone 30-F11 reacts with all isoforms and both CD45.1 and CD45.2 alloantigens of CD45.

Additional reported applications (for relevant formats) include: immunoprecipitation3, complement-dependent cytotoxicity1,5, immunohistochemistry (acetone-fixed frozen sections, zinc-fixed paraffin-embedded sections and formalin-fixed paraffin-embedded sections)4,6 and Western blotting7. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 103163 and 103164).

Application References

(PubMed link indicates BioLegend citation)
  1. Podd BS, et al. 2006. J. Immunol. 176:6532. (FC, CMCD) PubMed
  2. Haynes NM, et al. 2007. J. Immunol. 179:5099. (FC)
  3. Ledbetter JA, et al. 1979. Immunol. Rev. 47:63. (IP)
  4. Simon DI, et al. 2000. J. Clin. Invest. 105:293. (IHC)
  5. Seaman WE. 1983. J. Immunol. 130:1713. (CMCD)
  6. Cornet A, et al. 2001. P. Natl. Acad. Sci. USA 98:13306. (IHC)
  7. Tsuboi S and Fukuda M. 1998. J. Biol. Chem. 273:30680. (WB) PubMed
  8. Liu F, et al. 2012. Blood. 119:3295. PubMed
  9. Pelletier AN, et al. 2012. J. Immunol. 188:5561. PubMed
Product Citations
  1. DeFalco T, et al. 2014. Proc Natl Acad Sci U S A. 111:2384. PubMed
  2. Nikolaou K, et al. 2014. EMBO J. 34:430. PubMed
  3. Cha J, et al. 2015. J Biol Chem. 290:15337. PubMed
  4. Gereke M, et al. 2012. J Vis Exp. 70: 4322. PubMed
  5. Zareie P, et al. 2017. J Neuroinflammation. 0.630555556. PubMed
  6. Hernández-Santana YE, et al. 2020. Life Sci Alliance. 3:00. PubMed
  7. Claveria-Cabello A, et al. 2020. Cancers (Basel). 12:00. PubMed
  8. Li X, et al. 2020. Cell Rep. 31:107765. PubMed
  9. Fu H, et al. 2020. Front Immunol. 11:595813. PubMed
  10. Li Y, et al. 2020. Elife. 9:00. PubMed
  11. Parlanti P, et al. 2020. Exp Eye Res. 194:107998. PubMed
  12. Hagan AS, et al. 2020. Development. 147:00:00. PubMed
  13. Karhadkar TR, et al. 2021. PLoS One. 16:e0245924. PubMed
  14. Chen Q, et al. 2021. STAR Protocols. 2(1):100241. PubMed
  15. Chamberlin T, et al. 2020. Cancer Research. 80(20):4465-4475. PubMed
  16. Mori Y, et al. 2021. Diabetes and Vascular Disease Research. 18(3):14791641211027324. PubMed
  17. Kenswil KJG, et al. 2021. Cell Stem Cell. 28(4):653-670.e11. PubMed
  18. Altshuler A, et al. 2021. Cell Stem Cell. 28(7):1248-1261.e8. PubMed
  19. Huppé CA, et al. 2018. Mucosal Immunol. 0.536111111. PubMed
  20. Davies M, et al. 2017. PLoS Pathogens. 13(6):e1006435. PubMed
  21. Cédile O, Wlodarczyk A, and Owens T 2017. APMIS.. 10.1111/apm.12740. PubMed
  22. Peralta Ramos JM, et al. 2017. Front Immunol. 1.490277778. PubMed
  23. Sutton C, et al. 2017. Nat Commun.. 10.1038/s41467-017-02111-0. PubMed
  24. Seehus CR, et al. 2017. Nat Commun. 8:1900. PubMed
  25. Rhee S, et al. 2018. Nat Commun. 9:368. PubMed
  26. Mrdjen D et al. 2018. Immunity. 48(2):380-395 . PubMed
  27. Winter C, et al. 2018. Cell Metab. 28:175. PubMed
  28. Jones KB et al. 2018. Cell stem cell. 24(1):183-192 . PubMed
  29. Murai K et al. 2018. Cell stem cell. 23(5):687-699 . PubMed
  30. Moon H et al. 2017. Cell stem cell. 21(5):665-678 . PubMed
  31. Starossom SC, et al. 2019. Nat Commun. 10:217. PubMed
  32. Zabaleta N, et al. 2018. Nat Commun. 9:5454. PubMed
  33. Gao J, et al. 2017. Oncol Lett. 14:2954. PubMed
  34. Baluk P, et al. 2018. Methods Mol Biol. 1846:161. PubMed
  35. Emrick JJ, et al. 2018. Proc Natl Acad Sci U S A. 115:E12091. PubMed
  36. Krenzlin H, et al. 2019. J Clin Invest. 130:1671. PubMed
  37. Finn J et al. 2019. Cell reports. 26(11):2942-2954 . PubMed
  38. Stokes KL, et al. 2019. Oncogenesis. 8:24. PubMed
  39. Vu LT, et al. 2019. J Extracell Vesicles. 8:1599680. PubMed
  40. Deng W et al. 2019. Cell reports. 27(6):1755-1768 . PubMed
  41. Moon H, et al. 2019. Nat Commun. 10:2225. PubMed
  42. Thulasingam S, et al. 2019. Int J Mol Sci. 20. PubMed
  43. Nagatake T, et al. 2018. J Allergy Clin Immunol. 142:470. PubMed
  44. Axelrod HD, et al. 2019. Mol Cancer Res. 17:356. PubMed
  45. Bernard S, et al. 2018. J Mammary Gland Biol Neoplasia. 23:249. PubMed
  46. Haber AL, et al. 2017. Nature. 551:333. PubMed
  47. Schneider H, et al. 2017. J Neurochem. 140:170. PubMed
  48. Widjaja AA, et al. 2019. Gastroenterology. 157:777. PubMed
  49. Zhu F, et al. 2019. J Immunol. 203:2644. PubMed
  50. Janela B, et al. 2019. Immunity. 50:1069. PubMed
  51. Li Y et al. 2019. Endocrinology. 160(4):938-946 . PubMed
  52. Morita S et al. 2017. Cell metabolism. 25(4):883-897 . PubMed
  53. He X, et al. 2017. Cancer Biol Ther. 0.815277778. PubMed
  54. Rieck M, et al. 2017. Eur J Immunol. 47:677. PubMed
  55. Shafer MER, et al. 2017. Stem Cell Reports. 1.040277778. PubMed
  56. Ajona D, et al. 2017. Cancer Discov. 0.773611111. PubMed
  57. Fino KK, et al. 2017. Sci Rep. 5.334722222. PubMed
  58. Goel S, et al. 2017. Nature. 548:471. PubMed
  59. Poulos MG, et al. 2017. J Clin Invest. 127:4163. PubMed
  60. Nolin JD, et al. 2017. JCI Insight. 2:e94929. PubMed
  61. Burton OT, et al. 2018. Clin Exp Allergy. 48:288. PubMed
  62. Hoves S, et al. 2018. J Exp Med. 215:859. PubMed
  63. Pushalkar S, et al. 2018. Cancer Discov. 0.613194444. PubMed
  64. Diao J, et al. 2018. J Immunol. 201:1306. PubMed
  65. Sheppard S, et al. 2018. Front Immunol. 1.630555556. PubMed
  66. McLeod RL, et al. 2018. Oncotarget. 9:34459. PubMed
  67. Yoshihara S, et al. 2019. Front Immunol. 0.545833333. PubMed
  68. Arnold IC, et al. 2019. PLoS Pathog. 15:e1007866. PubMed
  69. Leist SR, et al. 2019. PLoS One. 14:e0220126. PubMed
  70. Rothchild AC, et al. 2019. Sci Immunol. 4:eaaw6693. PubMed
  71. Fiege JK, et al. 2019. PLoS Pathog. 15:e1008077. PubMed
  72. Langer V, et al. 2019. J Clin Invest. 129:4691. PubMed
  73. Wolf Y, et al. 2017. J Exp Med. 214:905. PubMed
  74. Roussey JA, et al. 2017. J Immunol. 199:3535. PubMed
  75. Chao Y, et al. 2020. Sci Adv. 6:eaaz4204. PubMed
  76. Davies CL, et al. 2019. Front Immunol. 10:1048. PubMed
  77. Chen X, et al. 2019. Methods Mol Biol. 2048:131. PubMed
  78. Lee YJ, et al. 2018. Front Microbiol. 9:83. PubMed
  79. Zhang J, et al. 2019. Onco Targets Ther. 12:4985. PubMed
  80. Shao Y, et al. 2017. Onco Targets Ther. 10:2675. PubMed
  81. Wang W, et al. 2018. Cancer Cell. 34:757. PubMed
  82. Tsubaki T, et al. 2018. Oncotarget. 9:11209. PubMed
  83. Hitchcock JR, et al. 2020. FEBS J. 287:250. PubMed
  84. Silvestre-Roig C, et al. 2019. Nature. 569:236. PubMed
  85. Seelige R, et al. 2018. Sci Rep. 8:13670. PubMed
  86. Ito S, et al. 2020. PLoS One. 15:e0229888. PubMed
  87. Qiu X, et al. 2018. Lab Chip. 18:2776. PubMed
  88. Paharik AE, et al. 2017. Cell Host Microbe. 22:746. PubMed
  89. Bar O, et al. 2020. Fluids Barriers CNS. 17:27. PubMed
  90. Mensurado S, et al. 2018. PLoS Biol. 16:e2004990. PubMed
  91. Kim D, et al. 2019. Immune Netw. 19:e32. PubMed
  92. Tamburini BAJ, et al. 2019. Front Immunol. 10:1036. PubMed
  93. Rincón E, et al. 2017. Oncotarget. 8:45415. PubMed
  94. Heinrich A, et al. 2020. Cell Rep. 31:107513. PubMed
  95. Vitiello GA, et al. 2018. Clin Cancer Res. 24:972. PubMed
  96. Paulo E, et al. 2018. Sci Rep. 8:11001. PubMed
  97. Lee S, et al. 2020. Cell Host & Microbe. 27(6):937-949. PubMed
  98. Melchor SJ, et al. 2020. Sci Rep. 10:15724. PubMed
  99. Arnold IC, et al. 2018. J Exp Med. 215:2055. PubMed
  100. Murad JP, et al. 2018. Front Immunol. 1.95. PubMed
  101. Matsuo K, et al. 2018. J Invest Dermatol. 138:1764. PubMed
  102. Podd B, et al. 2006. J Immunol. 176:6532. PubMed
  103. Latasa M, et al. 2010. PLoS One. 5:e15690. PubMed
AB_312966 (BioLegend Cat. No. 103101)
AB_312967 (BioLegend Cat. No. 103102)

Antigen Details

Protein tyrosine phosphatase (PTP) family, 180-240 kD

All hematopoietic cells except mature erythrocytes and platelets

Phosphatase, T and B cell activation
Galectin-1, CD2, CD3, CD4, TCR, CD22, Thy-1
Cell Type
B cells, Dendritic cells, Mesenchymal Stem Cells, Tregs
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.

Gene ID
19264 View all products for this Gene ID
View information about CD45 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 10/25/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Insert Note Here
Save Close Clear
Lab Timer
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account