Purified anti-mouse CD40 Antibody

Pricing & Availability
1C10 (See other available formats)
Regulatory Status
Other Names
Rat IgG2a, κ
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Product Citations
C57BL/6 mouse splenocytes stained with purified 1C10, followed by anti-rat IgG FITC
  • 1C10_Purified_080607
    C57BL/6 mouse splenocytes stained with purified 1C10, followed by anti-rat IgG FITC
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102802 500 µg £161
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CD40 is a 48 kD type I transmembrane glycoprotein, also known as Bp50. It is a member of the tumor necrosis factor receptor (TNFR) superfamily and is expressed on B cells, basal epithelial cells, macrophages, follicular dendritic cells, endothelial cells, and a subset of CD34+ hematopoietic progenitors. CD40 regulates B cell development/maturation, Ig isotype switching and, in combination with other signals such as IL-4, protects B cells from surface Ig-induced apoptosis and promotes proliferation. Interaction of CD40 with its ligand CD154 (gp39), which is expressed on activated T cells, is important in costimulation and immune regulation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Antibody Type
Host Species
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
The antibody was purified by affinity chromatography.
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.

FC - Quality tested
Costim, Block, IP - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The 1C10 antibody has been reported to stimulate B cell proliferation and block CD40-CD40L interactions. Additional reported applications (for the relevant formats) include: immunoprecipitation, blocking5,6 of CD40 ligand binding, and in vitro stimulation of B cell proliferation1-3. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 102810). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 102812) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

Application References

(PubMed link indicates BioLegend citation)
  1. Ferlin WG, et al. 1998. Eur. J. Immunol. 28:525. (FC, Costim)
  2. Ferlin WG, et al. 1996. Eur. J. Immunol. 26:2911. (Costim)
  3. Heath A, et al. 1994. Eur. J. Immunol. 24:1828. (Costim)
  4. Ashman RF, et al. 2005. Int. Immunol. 17:411.
  5. Pelßez B, et al. 2001. J. Immunol. 166:6608. (Block)
  6. Zhang P, et al. 2007. J. Immunol. 178:474. (Block)
  7. Lawson BR, et al. 2007. J. Immunol. 178:5366.
  8. Ponomarev ED, et al. 2007. J.Immunol.178:39. PubMed
Product Citations
  1. Chi H, et al. 2022. Cells. 11: . PubMed
  2. Obieglo K, et al. 2019. Eur J Immunol. 49:1226. PubMed
  3. Zhang P, et al. 2007. J Immunol. 178:474. PubMed
  4. Prado C, et al. 2021. J Neuroinflammation. 18:292. PubMed
  5. Zhang Y, et al. 2020. Front Immunol. 11:1012. PubMed
  6. Ashman R, et al. 2005. Int Immunol. 0.99375. PubMed
  7. Hoves S, et al. 2018. J Exp Med. 215:859. PubMed
  8. Le Q, Maizels N 2015. PLoS One. 11: 1005411. PubMed
  9. Patel J, et al. 2022. Int J Biol Sci. 18:2670. PubMed
  10. Sakaguchi T, et al. 2020. Cell Reports. 31(10):107755. PubMed
  11. Graf R et al. 2019. Cell reports. 26(5):1098-1103 . PubMed
  12. Lu X, et al. 2019. Cell Rep. 28:472. PubMed
  13. Rastelli J, et al. 2008. Blood. 111:1448. PubMed
  14. Khameneh HJ, et al. 2017. J Immunol. 198:196. PubMed
AB_312935 (BioLegend Cat. No. 102802)

Antigen Details

TNFR superfamily, 48 kD

B cells, basal epithelial cells, macrophages, follicular dendritic cells, endothelial cells, CD34+ hematopoietic progenitors

Stimulates B cell growth, differentiation and isotype switching, stimulates macrophage and dendritic cell cytokine production
CD40L (CD154)
Cell Type
B cells, Dendritic cells, Endothelial cells, Epithelial cells, Hematopoietic stem and progenitors, Macrophages
Biology Area
Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Costimulatory Molecules
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Bancherou J, et al. 1994. Annu. Rev. Immunol. 12:881.
3. Clark EA, et al. 1996. P. Natl. Acad. Sci. USA 83:4494.

Gene ID
21939 View all products for this Gene ID
View information about CD40 on UniProt.org

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Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.


This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.


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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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