Purified anti-mouse CD163 Antibody

Pricing & Availability
Clone
S15049I (See other available formats)
Other Names
M130, MM130, SCARI1
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
S15049I_Pure_CD163_Antibody_1_043018
C57BL/6 mouse peritoneal macrophages were cultured for seven days in the presence of Dexamethasone, then stained with CD11b APC and purified CD163 (clone S15049I, left), or purified rat IgG2a, κ isotype control (right), followed by PE Goat anti-rat IgG antibody.
  • S15049I_Pure_CD163_Antibody_1_043018
    C57BL/6 mouse peritoneal macrophages were cultured for seven days in the presence of Dexamethasone, then stained with CD11b APC and purified CD163 (clone S15049I, left), or purified rat IgG2a, κ isotype control (right), followed by PE Goat anti-rat IgG antibody.
  • S15049I_Pure_CD163_Antibody_2_043018
    C57BL/6 bone marrow were stained with CD11b APC and purified CD163 (clone S15049I, left), or purified rat IgG2a, κ isotype control (right), followed by PE Goat anti-rat IgG antibody. Data shown was gated on total bone marrow cells.
Cat # Size Price Quantity Avail. Save
155302 500 µg £168
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Description

CD163 is a member of the group B scavenger receptor cysteine-rich superfamily, also known as GHI/61, M130, RM3/1, p155, hemoglobin-haptoglobin complex receptor, or macrophage-associated antigen. It is a 134 kD (non-reduced)/155 kD (reduced) glycoprotein primarily expressed on macrophages, Kupffer cells, monocytes, a subset of dendritic cells, and a subset of hematopoietic stem/progenitor cells. CD163 binds to haptoglobin-hemoglobin complex and TWEAK, and plays a role in clearing hemoglobin and regulating cytokine production by macrophages. Membrane CD163 can be cleaved by metalloproteinases (MMP), resulting in a soluble form. Elevated serum level of sCD163 has been implicated in many kinds of inflammatory diseases.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant mouse CD163 extracellular domain
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2734239 (BioLegend Cat. No. 155302)

Antigen Details

Structure
Type 1 transmembrane glycoprotein, 134 kD (non-reduced)/155 kD (reduced) glycoprotein
Distribution

Macrophages, Kupffer cells, monocytes, a subset of dendritic cells, and a subset of hematopoietic stem/progenitor cells

Function
Binds to haptoglobin-hemoglobin complex and TWEAK, and plays a role in clearing hemoglobin and regulating cytokine production by macrophages
Biology Area
Immunology
Molecular Family
CD Molecules
Gene ID
93671 View all products for this Gene ID
UniProt
View information about CD163 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 05/01/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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