- A16070K (See other available formats)
- Other Names
- Monocyte chemotactic protein 2, MCP-2, small inducible cytokine subfamily A, member 8 (SCYA8),Chemokine (C-C motif) ligand 8, CCL8, MCP2
- Rat IgG2b, κ
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- Product Citations
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CCL8, also known as MCP-2, is a CC chemokine of the monocyte chemoattractant protein (MCP) family that plays key roles in allergic and inflammatory responses. CCL8 (MCP-2) has structural and functional similarity to other family members MCP-1 and MCP-3, and is often co-expressed with these chemokines in mononuclear cells. CCL8 is also constitutively expressed in the skin and lymph nodes of mice.
Mouse CCL8 induces a chemotactic migration of a population of differentiated Th2 cells enriched in IL-5 and IL-25 receptors by binding to the CCR8 receptor. Recent studies have established CCL8 as a critical ligand for driving CCR8-dependent Th2 cell trafficking into the skin, which in conjunction with locally recruited eosinophils drives chronic cutaneous allergic inflammation. CCL8 expression by suprabasal buldge cells has also been experimentally shown to negatively regulate the population of mouse hair follicles by Langerhans cells, further underscoring this chemokines central contributions to maintaining cutaneous immunity.
- Antibody Type
- Host Species
- Recombinant protein
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
ELISA Capture - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by ELISA assay. For ELISA Capture applications, a concentration range of 0.5 - 2 µg/mL is recommended. To obtain a linear standard curve, serial dilutions of CCL8 (MCP-2) recombinant protein ranging from 15.6 to 1,000 pg/mL is recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
The purified A16070K antibody is useful as the capture antibody in a sandwich ELISA assay, when used in conjunction with the biotinylated Poly5352 antibody (Cat. No. 535203) as the detection antibody and Recombinant Mouse CCL8 (MCP-2) (ELISA Std.) (Cat. No. 581709)
AB_2783344 (BioLegend Cat. No. 536902)
Fibroblast, endothelial cells, monocytes, macrophages, and skeletal muscle cells.
- CCL8 chemoattracts monocytes, T cells, NK cells, mast cells, eosinophils, and basophils. CCL8 is induced by IL-1β, IFNβ, IFNγ, dsRNA, virus, ConA, and anti-CD2. The induction of CCL8 by IFNγ synergizes with the TLR ligands peptidoglycan (TLR2), dsRNA (TLR3), and LPS (TLR4).
- Monocytes, T cells, NK cells, mast cells, eosinophils, and basophils.
- Ligand Receptor
- CCR1 (CD191), CCR2 (CD192), CCR3 (CD193), and CCR5 (CD195)
- Cell Type
- Endothelial cells, Fibroblasts, Macrophages, Monocytes
- Biology Area
- Cell Biology, Neuroinflammation, Neuroscience
- Molecular Family
- Antigen References
- Van Damme J, et al. 1992. J. Exp. Med. 176:59.
- Proost P, et al. 1998. J. Immunol. 160:4034.
- Dean RA, et al. 2008. Blood 112: 3455.
- Hori T, et al. 2008. Blood 111:4403.
- Rom S, et al. 2010. FASEB J. 24:2292.
- Struyf S, et al. 2009. Eur. J. Immunol. 39:843.
- Islam SA, et al. 2011. Nat. Immunol. 12:167.
- Gene ID
- 20307 View all products for this Gene ID
- View information about CCL8 on UniProt.org
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