Purified anti-MMP9 Antibody

Pricing & Availability
Clone
M2101G05 (See other available formats)
Regulatory Status
RUO
Other Names
Gelatinase B, Type IV collagenase, 92 kD gelatinase, Type V collagenase, GELB, CLG4B, MMP-9, MANDP2
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
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681802 100 µg £133.00
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Description

MMP9, or MMP-9, is a member of the matrix metalloprotease family of proteins (MMPs). Members of this family are structurally related, zinc-containing enzymes that degrade the extracellular matrix (ECM) and connective tissue proteins. Proteolytic activities of MMPs play an important role in vascular remodeling, cellular migration, and the processing of ECM proteins and adhesion molecules. MMP9 consists of a prodomain, catalytic domain, hinge region, and a hemopexin domain. It is secreted as an inactive zymogen that becomes activated extracellularly. The most relevant natural activators of proMMP9 are unknown. MMP9 activation may be mediated by removal of the prodomain by serine proteases or other MMPs, or it may be a direct response to oxidative stress that disrupts the cysteine switch. MMP9 is capable of processing cytokines and chemokines. For example, it is reported to release the biologically active form of vascular endothelial growth factor (VEGF), which indicates that MMP9 may play a crucial role in the formation of new blood vessels. Increased MMP9 expression and activity has been shown to be related to various cancers, as it may contribute to the release of metastatic cells from the bulk tumor as well as their entrance at the site of metastasis by degrading proteins in the ECM and basement membrane.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human MMP-9, amino acids (Ala20-Asp707) (Accession# NP_004985.2), is expressed with a C-terminal 8His tag and a linker sequence in the 293E cell line.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ELISA Capture - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA Capture applications, a concentration of 4.0 µg/mL is recommended. To obtain a linear standard curve, serial dilutions of MMP-9 recombinant protein ranging from 5 to 0.078 ng/mL is recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is only tested on recombinant proteins. It may not be able to detect endogenous proteins from cell or tissue lysates by western blotting (WB), where the expression levels may be too low.

The purified M2101G05 antibody is useful as the capture antibody in a sandwich ELISA assay, when used in conjunction with the biotinylated M2108F07 antibody (Cat. No. 532101) as the detecting antibody.

Note: For testing human MMP-9 in serum or plasma, BioLegend's LEGEND MAX™ Kit (Cat. No. 444907) is specially developed and recommended.

Product Citations
RRID
AB_2566497 (BioLegend Cat. No. 681802)

Antigen Details

Distribution

MMP9 is expressed by alveolar macrophages, granulocytes, fibroblasts, and fibrosarcoma cells.

Function
Degradation of extracellular matrix (ECM) and connective tissue proteins, angiogenesis, tissue remodeling, and cancer metastasis. Regulates chemokine activity. Inhibited by TIMPs and α2-macroglobulin (inhibitor of MMPs in bodily fluids).
Interaction
ECM proteins.
Ligand/Receptor
TIMPs.
Cell Type
Fibroblasts, Granulocytes, Macrophages
Biology Area
Angiogenesis, Cell Adhesion, Cell Biology, Neuroinflammation, Neuroscience
Molecular Family
Enzymes and Regulators
Antigen References

1. Chakrabarti S, Patel KD. 2005. Exp. Lung Res. 31:599.
2. Klein T, Bischoff R. 2011. Amino Acids 41:271.
3. Raffetto JD, Khalil RA. 2008. Biochem. Pharmacol. 75:346.
4. Bourboulia D, Stetler-Stevenson WG. 2010. Semin. Cancer Biol. 20:161.
5. Vilen ST, et al. 2013. Scientific World Journal 920595.

Gene ID
4318 View all products for this Gene ID
UniProt
View information about MMP-9 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 05/31/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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