- W18219A (See other available formats)
- Other Names
- Methyltransferase Like 14, N6-Adenosine-Methyltransferase Non-Catalytic Subunit, HMETTL14, KIAA1627
- Rat IgG2a, κ
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- Product Citations
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METTL14 forms a heterodimer with METTL3 called the m6A-methyltransferase complex (MTC) to methylate adenosine residues at the N6 position of eukaryotic mRNAs. MTC-induced methylation takes place at the 5'-[AG]GAC-3' consensus sites of some mRNAs. In the MTC complex, METTL14 constitutes the RNA-binding scaffold that recognizes the substrate. Once methylated, the mRNAs are recognized by distinct proteins known as “readers” that will affect their stability, splicing, and/or translation.Product Details
- Human, Mouse
- Antibody Type
- Host Species
- Partial-length recombinant human METTL14 protein between 1-300 a.a.
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
WB - Quality tested
IP - Validated
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.025 - 1.0 µg per ml. For immunoprecipitation, the suggested use of this reagent is 2.5 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
While this clone is able to immunoprecipitate METTL14 from whole cell extracts, the resulting fraction was not significantly enriched in METTL14; therefore, this clone may not be suitable for detecting transient or low-abundance complexes.
- METTL14 is a 456 amino acid protein with a molecular weight of 65 kD.
Localized to the mitochondria; ubiquitously expressed.
- Methylation; mRNA splicing; mRNA translation
- Biology Area
- Protein Synthesis
- Antigen References
- Liu J, et al. 2014. Nat. Chem. Biol. 10.1038/nchembio.1432.
- Nagase T, et al. 2000. DNA Res. 7:273-81.
- Yue Y, et al. 2018. Cell Discov. 4:10.
- Schöller E, et al. 2018. RNA. 10.1261/rna.064063.117.
- Wang X, et al. 2016. Nature. 10.1038/nature18298.
- Gene ID
- 57721 View all products for this Gene ID
- View information about METTL14 on UniProt.org
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