Purified anti-Loricrin Antibody

Pricing & Availability
Clone
Poly19051 (See other available formats)
Other Names
Loricrin
Isotype
Rabbit Polyclonal IgG
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Product Citations
publications
1_Poly19051_PURE_Loricrin_Antibody_1_IHC_052716
IHC staining of anti-Loricrin antibody (Poly19051) on formalin-fixed paraffin-embedded human skin tissue. Following antigen retrieval using Sodium Citrate, the tissue was incubated with the primary antibody at 1 µg/ml for 60 minutes at room temperature. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit was used for detection followed by hematoxylin counterstaining, according to the protocol provided.
  • 1_Poly19051_PURE_Loricrin_Antibody_1_IHC_052716
    IHC staining of anti-Loricrin antibody (Poly19051) on formalin-fixed paraffin-embedded human skin tissue. Following antigen retrieval using Sodium Citrate, the tissue was incubated with the primary antibody at 1 µg/ml for 60 minutes at room temperature. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit was used for detection followed by hematoxylin counterstaining, according to the protocol provided.
  • 2_Poly19051_PURE_Loricrin_Antibody_1_WB_120417
    Total lysates (15 µg protein) from HaCaT (human, positive control) and U87-MG (human, negative control) cells were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 1:500 (1 µg/ml) purified anti-Loricrin antibody, clone Poly19051. Proteins were visualized using chemiluminescence detection by incubation with HRP Donkey anti-Rabbit secondary antibody (Cat. No. 406401, 1:3000 dilution). Direct-Blot™ HRP anti-β-actin antibody was used as a loading control (Cat. No. 643807, 1:8000 dilution).
  • 3_Poly19051_PURE_Loricrin_Antibody_2_WB_052716
    Western blot of anti-Loricrin antibody (Poly19051). Lane 1: Molecular weight marker; Lane 2: Human skin lysate. The blot was incubated with the primary antibody at 2 µg/ml for 24 hours at 4°C, followed by incubation with an HRP goat anti-rabbit IgG secondary antibody and chemiluminescence detection.
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905103 25 µg £50
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905104 100 µg £165
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Description

Loricrin is a major protein component of the cornified cell envelope found in terminally differentiated epidermal cells. Mutations in this gene are associated with Vohwinkel's syndrome and progressive symmetric erythrokeratoderma, both inherited skin diseases.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
This antibody was raised against a peptide sequence derived from the C-terminus of the mouse loricrin protein.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
IHC-F, WB - Validated
IF - Reported in the literature

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded (FFPE) immunohistochemical staining. For immunohistochemisty of FFPE and frozen sections, a concentration range of 0.1 - 10 μg/ml is suggested. For Western blotting, a usage of 1.0 μg/ml (1:500) is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Predicted MW is 26 kD (Human), 38 kD (Mouse). Observed MW for human is approximately 35 kD.

This product may contain other non-IgG subtypes.

Application References

(PubMed link indicates BioLegend citation)
  1. Suzuki K, et al. 2009. Development 136:367. (IF) PubMed
Product Citations
  1. Moriyama M, et al. 2016. PLoS One. 11:e0164799. PubMed
  2. Nishiguchi MA, et al. 2018. Cell Rep. 24:3383. PubMed
  3. Moon H, et al. 2019. Nat Commun. 10:2225. PubMed
  4. Zhang Z, et al. 2018. Nat Med. 24:617. PubMed
Publication Library
RRID
AB_2734676 (BioLegend Cat. No. 905103)
AB_2616895 (BioLegend Cat. No. 905104)

Antigen Details

Structure
Loricrin is a 312 amino acid protein with a molecular mass of 26 kD.
Distribution

Tissue distribution: skin.
Cellular distribution: nucleus and plasma membrane.

Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure
Antigen References

1. Wallace L. 2012. J. Cell Sci. 125:1750. (IHC, WB) PubMed
2. Bayo P, et al. 2008. Endocrinology 149:1377. (IHC) PubMed
3. Hu Y, et al. 2001. Nature 410:710. PubMed
4. Croyle MJ, et al. 2011. Development 138:1675. (IF) PubMed
5. Sengupta A, et al. 2010. PLoS One 5(8):e12249. (IHC) PubMed
6. Liakath-Ali K, et al. 2014. Nat. Commun. 5:3540. (IF) PubMed

Gene ID
4014 View all products for this Gene ID
UniProt
View information about Loricrin on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 12/04/2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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