Purified anti-LHX6.1 Antibody

Pricing & Availability
Clone
N228A/16 (See other available formats)
Other Names
LHX6, LHX6.1
Isotype
Mouse IgG1, κ
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Product Citations
publications
N228A-16_Purified_LHX6-1_Antibody_1_112018
IHC staining of purified anti-LHX6.1 antibody (clone N228A/16) on formalin-fixed paraffin-embedded human kidney tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • N228A-16_Purified_LHX6-1_Antibody_1_112018
    IHC staining of purified anti-LHX6.1 antibody (clone N228A/16) on formalin-fixed paraffin-embedded human kidney tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • N228A-16_Purified_LHX6-1_Antibody_2_112018
    IHC staining of purified anti-LHX6.1 antibody (clone N228A/16) on formalin-fixed paraffin-embedded mouse kidney tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928602), the tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
Cat # Size Price Quantity Avail. Save
857301 25 µg £47
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857302 100 µg £94
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Description

LHX6 is a transcription factor that belongs to the LIM-homeobox protein family. LHX6 contains two LIM domains, followed by one homeodomain (HD). The protein has been shown to play an important role during neurodevelopment where it functions as a cell fate regulator that controls differentiation and development of neural cells, especially cholinergic and GABAergic neurons1. In the nervous system, LHX6 is mainly expressed in neurons, including motor neurons, interneurons, and sensory neurons.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2783471 (BioLegend Cat. No. 857301)
AB_2783472 (BioLegend Cat. No. 857302)

Antigen Details

Structure
LHX6 is a 363 amino acid protein with an apparent molecular mass of ~ 40 kD.
Distribution

Tissue Distribution: Ubiquitously expressed in all tissues.
Cellular Distribution: Nucleus

Function
LHX6 functions as a cell fate regulator that controls differentiation and development of neural cells.
Interaction
Forms homodimers or heterodimers with other LHX family members such as LHX8. Interacts with LDB1, PITX2, RLIM
Biology Area
Cell Biology, Neuroinflammation, Neuroscience, Neuroscience Cell Markers, Synaptic Biology
Molecular Family
Postsynaptic proteins
Antigen References
  1. Zhou C, et al. 2015 FASEB J. 29(10):4083
  2. Nathalia E, et al. 2018. Rom J Intern Med. 56(3):135
  3. Wullimann MF, et al. 2009. Integr Zool. 4(1):123
Gene ID
26468 View all products for this Gene ID 16874 View all products for this Gene ID
UniProt
View information about LHX6.1 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 11/21/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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