Purified anti-LEF1 Antibody

Pricing & Availability
Clone
W17155A (See other available formats)
Other Names
Lymphoid enhancer-binding factor 1, TCF1α, TCF10
Isotype
Rat IgG2b, κ
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Product Citations
publications
Total cell lysates (15 µg total protein) from A549 and HeLa cells (negative control), Jurkat, MOLT-4 and EL4 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-LEF1 Antibody, clone W17155A, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG Antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:10000 dilution (lower). Lane M: Molecular Weight marker. Predicted LEF1 expression data was obtained from Human Protein Atlas.
  • Total cell lysates (15 µg total protein) from A549 and HeLa cells (negative control), Jurkat, MOLT-4 and EL4 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-LEF1 Antibody, clone W17155A, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG Antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:10000 dilution (lower). Lane M: Molecular Weight marker. Predicted LEF1 expression data was obtained from Human Protein Atlas.
  • HeLa (negative control, panel A) and Jurkat (panel B) were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with ice-cold methanol for 10 minutes, and blocked with 5% FBS for 60 minutes. Cells were then intracellularly stained with a 1:100 dilution (5 µg/mL) of Purified LEF1 Antibody, Clone W17155A overnight at 4°C, followed by incubation with Alexa Fluor® 594 goat anti-rat IgG (Cat. No. 405422) at 2.0 µg/mL. Nuclei were counterstained with DAPI and the image was captured with a 60X objective.
  • Whole cell extracts (300 µg total protein) prepared from Jurkat cells were immunoprecipitated overnight with 2.5 µg of Purified Rat IgG2b, κ Isotype Control Antibody (ISO IP, Cat. No. 400602) or Purified anti-LEF1 Antibody, clone W17155A (W17155A IP). The resulting IP fractions and whole cell extract input (5%) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with a separate anti-LEF1 antibody raised with a different immunizing sequence. Lane M: Molecular Weight marker.
Cat # Size Price Quantity Avail. Save
616001 25 µg £57
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616002 100 µg £144
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Description

Lymphoid Enhancing Factor 1 (LEF1) is a transcription factor belonging to the TCF/LEF family. LEF1 was first identified as a protein binding to TCR-α enhancer and upregulates expression of T-cell antigen receptor α chain. LEF1 is involved in Wnt signaling pathways as a ubiquitous regulator. During Wnt signaling, translocation of β-catenin from the cytosol to the nucleus results in elevation of transcriptional activity of LEF1, which subsequently regulates multiple downstream genes.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partial recombinant human LEF1 protein corresponding to amino acid residues 116-283
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC, IP - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 µg per ml. For immunocytochemistry, a concentration of 5.0 μg/ml is recommended. For immunoprecipitation, the suggested use of this reagent is 2.5 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

W17155A recognizes multiple isoforms of LEF1.

When tested for ICC, W17155A failed to stain PFA-fixed Jurkat cells permeabilized with methanol. We recommend Triton X-100 permeabilization.

Antigen Details

Structure
LEF1 is a 399 amino acid protein with a predicted molecular weight of 44 kD. Seven total isoforms have been reported, ranging in size from 23 to 44 kD.
Distribution

Lymphoid tissue enriched/Nuclear localization

Function
Canonical Wnt signaling
Biology Area
Angiogenesis, Cell Biology, Immunology, Signal Transduction, Transcription Factors
Molecular Family
Nuclear Markers, TCRs
Antigen References

1. Mallory MJ, et al. 2011. Mol. Cell. Biol. 31:2184.
2. Waterman ML, et al. 1991. Genes Dev. 5:656.
3. Hovanes K, et al. 2001. Nat Genet. 28:53.
4. Bruhn L, et al. 1997. Genes Dev. 11:640.
5. Nguyen A, et al. 2005. Int. J. Oncol. 27:949.
6. Petropoulos K, et al. 2008. J. Exp. Med. 205:515.

Gene ID
51176 View all products for this Gene ID
UniProt
View information about LEF1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 07/05/2019

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