- LAT1111 (See other available formats)
- Regulatory Status
- Other Names
- Linker for activation of T-cells, 36 kD phospho-tyrosine adaptor protein
- Mouse IgG1, κ
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- Product Citations
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LAT (linker for activation of T-cells) is a 36 kD phospho-tyrosine adaptor protein containing SH2 domains and a CVRC motif. Two LAT isoforms have been reported with approximate molecular weights of 36 kD and 38 kD. LAT is localized to lipid rafts, and is also a potential type III transmembrane protein, thought to be expressed in the juxtanuclear intracellular compartment. LAT links TCR engagement to Ras-MAPK activation, Ca2+ flux, IL-2 expression and is required for T-cell development. LAT is phosphorylated when activated by TCR cross-linking, where it recruits signaling molecules. LAT activation can be prevented by polyunsaturated fatty acids. LAT is modified by ZAP-70 and Syk phosphorylation and can also be palmitoylated. LAT has been reported to be associated with Grb2, Gads, Grap, PLC-γ1, p85 subunit PI3K, ZAP-70, Syk, Itk, Vav, SLP-76, and cbl.Product Details
- Verified Reactivity
- Antibody Type
- Host Species
- Partial recombinant protein (cytoplasmic domain of human LAT)
- This LAT antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. Final antibody concentration is 0.5 mg/ml.
- The antibody was purified by affinity chromatography.
- 0.5 mg/ml
- Storage & Handling
- Upon receipt, store between 2°C and 8°C.
WB - Quality tested
ICFC - Verified
IP - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 µg antibody per 5 ml antibody dilution buffer for each mini-gel. For flow cytometric staining, the suggested use of this reagent is ≤0.06 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
The LAT1111 monoclonal antibody recognizes the cytoplasmic domain of human LAT and has been shown to be useful for Western blotting and immunoprecipitation. The LAT1111 antibody does not cross-react with mouse LAT.
- Product Citations
AB_2133119 (BioLegend Cat. No. 623901)
AB_2133118 (BioLegend Cat. No. 623902)
- SH2 domains, CVRC motif; two isoforms 36 kD, 38 kD
Lipid rafts, potential type III transmembrane protein, juxtanuclear intracellular compartment
- Links TCR engagement to Ras-MAPK activation, Ca2+ flux, IL-2 expression; required for T-cell development
- Grb2, Gads, Grap, PLC-γ1, p85 subunit PI3K, ZAP-70, Syk, Itk, Vav, SLP-76, cbl
- Phosphorylation by ZAP-70, Syk, Palmitoylated
- Cell Type
- Biology Area
- Cell Biology, Immunology, Signal Transduction
- Molecular Family
- Antigen References
1. Zhang W, et al. 1998. Cell. 92:83.
2. Zeyda M, et al. 2002. J. Biol. Chem. 277:28418.
3. Perez-Villar J, et al. 2002 Biochemistry. 41:10732.
4. Hartgroves L, et al. 2003 J. Biol. Chem. 278:20389.
- Phosphorylated when activated by TCR cross-linking, recruits signaling molecules, activation prevented by polyunsaturated fatty acids
- Gene ID
- 27040 View all products for this Gene ID
- View information about LAT on UniProt.org
|Purified anti-LAT||LAT1111||WB, ICFC, IP|
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