Purified anti-Keratin 5 Antibody

Pricing & Availability
Clone
Poly19055 (See other available formats)
Other Names
Keratin, type II cytoskeletal 5, CK-5, 58 kda cytokeratin, type-II keratin Kb5, epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types, keratin 5 (epidermolysis bullosa simplex, Dowling-Meara/Kobner/Weber-Cockayne types), krt5
Isotype
Rabbit Polyclonal IgG
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Product Citations
publications
Poly19055_PURE_Keratin5_Antibody_1_IHCP_062216
IHC staining of anti-Keratin 5 antibody (Poly19055) on formalin-fixed paraffin-embedded human skin tissue. Following antigen retrieval using Sodium Citrate, the tissue was incubated with the primary antibody at 0.2 µg/ml for 60 minutes at room temperature. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit was used for detection followed by hematoxylin counterstaining.
  • Poly19055_PURE_Keratin5_Antibody_1_IHCP_062216
    IHC staining of anti-Keratin 5 antibody (Poly19055) on formalin-fixed paraffin-embedded human skin tissue. Following antigen retrieval using Sodium Citrate, the tissue was incubated with the primary antibody at 0.2 µg/ml for 60 minutes at room temperature. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit was used for detection followed by hematoxylin counterstaining.
  • Poly19055_PURE_Keratin5_Antibody_2_WB_062216
    Total lysates (15 µg protein) from A431 (Human, Positive control), MCF-7 (Low expression negative control), Raw264.7 (Mouse) and UMR106 (Rat) were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 1:500 (1 µg/ml) purified Keratin 5 antibody, clone Poly19055. Proteins were visualized using chemiluminescence detection by incubation with HRP Donkey anti-Rabbit secondary antibody (Cat. No. 406401, 1:3000 dilution). GAPDH (Cat. No. 919501) was used as a loading control (Cat. No. 919501, 1:3000 dilution).
  • Poly19055_PURE_Keratin5_Antibody_3_ICC_062216
    Immunofluorescence of A431 cells with (A) Rabbit Isotype control (Negative, Cat. No. 910801) or (B-D) Keratin 5 primary antibody (Poly19055). Alexa Fluor® 488 (Green) Donkey anti-Rabbit IgG (Cat. No. 406416) was used as secondary antibody. Nuclei were counterstained with DAPI (Blue, Cat. No. 422801). The image was captured with a 60X objective using KEYENCE BZ-X700 fluorescence microscope. Exposure time (Seconds) for (A) is 1/10, and (B-D) is 1/25. Concentrations for (A, B) is 2 µg/ml, (C) is 1 µg/ml, (D) is 0.5 µg/ml.
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Cat # Size Price Quantity Avail. Save
905503 25 µg £50
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905504 100 µg £165
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Description

Keratin 5 is a member of the type II (basic or neutral) cytokeratin family. Type II keratins, in general, are heteropolymeric structural proteins coexpressed during differentiation of simple and stratified epithelial tissues. Keratin 5 is specifically expressed in the basal layer of the epidermis along with Keratin 14.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse, Rat, Cross Reactivity: Dog, Primate
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
This monospecific polyclonal antibody was raised against a peptide sequence derived from the C-terminus of the mouse keratin 5 protein.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
WB, ICC - Validated

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemisty, a concentration range of 0.2 - 5.0 μg/ml is suggested. For Western blotting, a concentration range of 0.5 - 1.0 μg/ml is suggested. For immunocytochemistry, a concentration range of 0.5 - 1.0 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Predicted MW is ~ 62 kD. This antibody weakly reacts with mouse.

 

This product may contain other non-IgG subtypes.

Application References

(PubMed link indicates BioLegend citation)
  1. Hübner A, et al. 2012. Proc. Natl. Acad. Sci. USA 109:12046. (IHC) PubMed
  2. Kierszenbaum AL, et al. 2003. Mol. Biol. Cell 14(11):4628. (IHC) PubMed
  3. Hu Y, et al. 2001. Nature 410:710.
  4. Yuspa SH, et al. 1989. J. Cell Biol. 109:1207.
  5. Liang Y. 2011. Patholog. Res. Int. 2011:936794. (IHC) PubMed
  6. Roop DR, et al. 1984. J. Biol. Chem. 259:8037.
Product Citations
  1. Zhussupbekova S, et al. 2016. EBioMedicine. 9: 314-23. PubMed
  2. Kim SE, et al. 2019. Anal Cell Pathol (Amst). 2018:8120579. PubMed
  3. Nagai-Okatani C, et al. 2019. Int J Mol Sci. 20:. PubMed
  4. Taylor MS, et al. 2018. Am J Pathol. 188:1149. PubMed
  5. Adams CR, et al. 2019. Elife. 8. PubMed
  6. Kim M, et al. 2019. Nat Commun. 10:3991. PubMed
Publication Library
RRID
AB_2734679 (BioLegend Cat. No. 905503)
AB_2616956 (BioLegend Cat. No. 905504)

Antigen Details

Structure
Keratin 5 is a 62 kD protein.
Distribution

Cytoskeleton, extracellular, and nucleus.

Function
Keratin 5 mutations have been associated with a complex of diseases termed epidermolysis bullosa simplex.
Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family
Intermediate Filaments
Gene ID
3852 View all products for this Gene ID
UniProt
View information about Keratin 5 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 06/22/2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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