Purified anti-Keratin 14 Antibody

Pricing & Availability
Clone
Poly19053 (See other available formats)
Other Names
Keratin, type I cytoskeletal 14, CK-14, cytokeratin 14, keratin 14 (epidermolysis bullosa simplex, Dowling-Meara, Koebner)
Isotype
Rabbit Polyclonal IgG
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Product Citations
publications
1_Poly19053_PURE_Keratin14_Antibody_1_WB_102317
Western blot analysis of cell lysates from HaCaT (Positive control) and U87-MG (Low expression negative control) cells using Keratin 14 Rabbit primary antibody and HRP Donkey anti-Rabbit secondary antibody (#406401). Direct-Blot™ HRP anti-β-actin (#643807) was used as a loading control.
  • 1_Poly19053_PURE_Keratin14_Antibody_1_WB_102317
    Western blot analysis of cell lysates from HaCaT (Positive control) and U87-MG (Low expression negative control) cells using Keratin 14 Rabbit primary antibody and HRP Donkey anti-Rabbit secondary antibody (#406401). Direct-Blot™ HRP anti-β-actin (#643807) was used as a loading control.
  • 2_Poly19053_PURE_Keratin14_Antibody_1_IHC_052716
    IHC staining of anti-Keratin 14 antibody (Poly19053) on formalin-fixed paraffin-embedded normal human skin. After antigen retrieval using Sodium Citrate, the tissue was incubated with the primary antibody at 1 µg/ml for 60 minutes at room temperature. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit was used for detection followed by hematoxylin counterstaining, according to the protocol provided.
  • 3_Poly19053_PURE_Keratin14_Antibody_2_WB_052716
    Western blot of anti-Keratin 14 antibody (Poly19053). Lane 1: Molecular weight marker; Lane 2: Human skin lysate. The blot was incubated with the primary antibody at 1 µg/ml, overnight at 4°C, followed by incubation with a fluorescent labeled goat anti-mouse secondary antibody.
  • Poly19053_PURE_Keratin-14_Antibody_ICC_112817
    Immunofluorescence of HaCaT cells with (A) Rabbit Isotype control (Cat. No. 910801) or (B-D) Keratin 14 primary Antibody (Clone Poly 19053). 2 µg/ml (1:250 dilution) Alexa Fluor® 594 (red) Donkey anti-Rabbit IgG (Cat. No. 406418) was used as secondary antibody. Nuclei were counterstained with DAPI (blue). The image was captured with a 60X objective using KEYENCE BZ-X700 fluorescence microscope. Exposure time (Seconds) for (A) is 1/30, and (B-D) is 1/60. Anti-Keratin 14 primary antibody concentrations for (A, B) is 2 µg/ml (1:250 dilution), (C) is 1 µg/ml (1:1000 dilution), (D) is 0.5 µg/ml (1:1000 dilution).
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Cat # Size Price Quantity Avail. Save
905303 25 µg £50
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905304 100 µg £165
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Description

Keratin 14 is a member of the keratin family, the most diverse group of intermediate filaments. This gene product, a type I keratin, is usually found as a heterotetramer with two keratin 5 molecules, a type II keratin. Together they form the cytoskeleton of epithelial cells. Mutations in the genes for these keratins are associated with epidermolysis bullosa simplex.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse, Rat; Cross Reactivity: Dog, Primate
Antibody Type
Polyclonal
Host Species
Rabbit
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
ICC, WB - Validated

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 0.1 - 10 μg/ml is suggested. For Western blotting, a concentration range of 0.5 - 2 ug/ml (1:250-1:1000) is suggested. For immunocytochemistry, a concentration range of 0.5 - 2.0 μg/ml (1:250 - 1:1000) is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes


This product may contain other non-IgG subtypes.

Application References

(PubMed link indicates BioLegend citation)
  1. Hu Y, et al. 2001. Nature 410:710. PubMed
  2. Yuspa SH, et al. 1989. J. Cell. Biol. 109:1207. PubMed
  3.  
Product Citations
  1. Altmeier S, et al. 2016. PLoS Pathog. 12: 1005882. PubMed
  2. Jones KB 2018. Cell stem cell. 24:183. PubMed
  3. Moehring F 2018. eLife. 7: e31684. PubMed
  4. Giraddi RR, et al. 2018. Cell Rep. 24:1653. PubMed
  5. Nishiguchi MA, et al. 2018. Cell Rep. 24:3383. PubMed
  6. Cowie AM, et al. 2018. J Neurosci. 38:5807. PubMed
  7. Changarathil G, et al. 2019. PLoS One. 14:e0215908. PubMed
  8. Adams CR, et al. 2019. Elife. 8. PubMed
  9. Fu R, et al. 2019. Nat Commun. 10:3210. PubMed
  10. Anderton H, et al. 2018. Cell Death Differ. 26:877. PubMed
  11. Anderton H, et al. 2017. J Invest Dermatol. 137:2371. PubMed
Publication Library
RRID
AB_2734678 (BioLegend Cat. No. 905303)
AB_2616896 (BioLegend Cat. No. 905304)

Antigen Details

Structure
Keratin 14 is a 472 amino acid protein with a molecular mass of 52 kD.
Distribution

Tissue distribution: skin.
Cellular distribution: extracellular, nucleus, cytoskeleton, and cytosol.

Function
Nonhelical tail domain is involved in promoting KRT5-KRT14 filaments to self-organize into large bundles, enhances the mechanical properties involved in resilience of keratin intermediate filaments in vitro.
Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family
Intermediate Filaments
Antigen References

1. Wend P, et al. 2013. EMBO J. 32(14):1977-89. PubMed
2. Welm AL, et al. 2005. Proc. Natl. Acad. Sci. USA 102(12):4324. (IHC) PubMed
3. Liang Y. 2011. Pathalog. Res. Int. 2011:93674. (IHC) PubMed

Gene ID
3861 View all products for this Gene ID
UniProt
View information about Keratin 14 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 4    Revision Date: 11/28/2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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