Purified anti-Keratin 1 Antibody

Pricing & Availability
Clone
Poly19052 (See other available formats)
Other Names
Keratin, type II cytoskeletal 1, CK-1, cytokeratin-1, 67 kD cytokeratin, hair alpha protein, type-II keratin Kb1, epidermolytic hyperkeratosis 1, krt1
Isotype
Rabbit Polyclonal
Ave. Rating
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Product Citations
publications
1_Poly19052_PURE_Keratin1_Antibody_3_WB_031519.png
Total cell lysates (15 µg total protein) from A549 and HEL (negative controls) and HaCaT (positive control) cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.25 µg/mL of Purified anti-KRT1 Antibody, clone Poly19052, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP donkey anti-rabbit IgG Antibody (Cat. No. 406401) at a 1:3000 dilution. Direct-Blot™ HRP anti-β actin Antibody (Cat. No. 664804) was used as a loading control at a 1:10000 dilution (lower). Lane M: Molecular Weight marker. Predicted KRT1 expression data obtained from Human Protein Atlas.
  • 1_Poly19052_PURE_Keratin1_Antibody_3_WB_031519.png
    Total cell lysates (15 µg total protein) from A549 and HEL (negative controls) and HaCaT (positive control) cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.25 µg/mL of Purified anti-KRT1 Antibody, clone Poly19052, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP donkey anti-rabbit IgG Antibody (Cat. No. 406401) at a 1:3000 dilution. Direct-Blot™ HRP anti-β actin Antibody (Cat. No. 664804) was used as a loading control at a 1:10000 dilution (lower). Lane M: Molecular Weight marker. Predicted KRT1 expression data obtained from Human Protein Atlas.
  • Poly19052_PURE_Keratin1_Antibody_1_IHCP_062816
    IHC staining of anti-Keratin 1 antibody (Poly19052) on formalin-fixed paraffin-embedded normal human skin. After antigen retrieval using sodium citrate, the tissue was incubated with the primary antibody at 1 µg/ml for 60 minutes at room temperature. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit was used for detection followed by hematoxylin counterstaining.
  • Poly19052_PURE_Keratin1_Antibody_2_WB_062816
    Western blot of anti-Keratin 1 antibody (Poly19052). Lane 1: Molecular weight marker; Lane 2: Human skin lysate. The blot was incubated with the primary antibody at 1 µg/ml for 24 hours at 4°C, followed by incubation with a goat anti-rabbit IgG secondary antibody conjugated to HRP and chemiluminescence detection.
See high resolution IHC-P data...
Cat # Size Price Quantity Avail. Save
905203 25 µg £50
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905204 100 µg £165
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Description

Keratin 1 is a member of the keratin family of proteins. It is a type II cytokeratin specifically expressed in the spinous and granular layers of the epidermis along with family member Keratin 10. Mutations in these genes have been associated with bullous congenital ichthyosiform erythroderma.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Rat, Cross-Reactivity: Primate
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
The antibody was raised against a peptide sequence derived from the C-terminus of the human keratin 1 protein.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
IHC-F, WB - Validated

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 0.1 - 10 μg/ml is suggested. For Western blotting, a concentration range of 0.25 - 1.0 μg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes


This product may contain other non-IgG subtypes.

Application References

(PubMed link indicates BioLegend citation)
  1. Wu N, et al. 2012. J. Biol. Chem. 287:5627. (WB) PubMed
  2. Schütz M, et al. 2011. Hum. Mol. Genet. 20:28. (IHC) PubMed
  3. Hu Y, et al. 2001. Nature 410:710. PubMed
  4. Yuspa SH, et al. 1989. J. Cell Biol. 109:1207. PubMed
  5. Roop DR, et al. 1984. J. Biol. Chem. 259:8037. PubMed
Product Citations
  1. Noguchi S, et al. 2019. Commun Biol. 2:37. PubMed
  2. Xu M, et al. 2019. Am J Transl Res. 11:257. PubMed
Publication Library
RRID
AB_2734677 (BioLegend Cat. No. 905203)
AB_2616954 (BioLegend Cat. No. 905204)

Antigen Details

Structure
Keratin 1 is a 66 kD protein.
Distribution

Extracellular, nucleus, and cytoskeleton.

Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family
Intermediate Filaments
Gene ID
3848 View all products for this Gene ID
UniProt
View information about Keratin 1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 03/15/2019

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