- 12A4A35 (See other available formats)
- Regulatory Status
- Other Names
- Interferon regulatory factor-3 (IRF-3)
- Mouse IgG2b, κ
- Ave. Rating
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- Product Citations
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IRF-3 is a member of the interferon regulatory transcription factor (IRF) family. It mediates interferon-stimulated response element (ISRE) promoter activation and functions as a molecular switch for antiviral activity. DsRNA generated during the course of a viral infection leads to IRF-3 phosphorylation on the C-terminal serine/threonine cluster. This induces a conformational change, leading to its dimerization, nuclear localization and association with CREB protein binding to dsRNA-activated factor 1, a complex which activates the transcription of genes under control of ISRE. The phosphorylated IRF-3 is under negative regulation by ubiquitin-mediated degradation. Research indicates that it plays a critical role in stimulated TLR-mediated IL-27 p28 gene expression.Product Details
- Human, Mouse
- Antibody Type
- Host Species
- Partial IRF-3 recombinant protein (200-427 a.a.)
- This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Affinity purified
- 0.5 mg/ml
- Storage & Handling
- Upon receipt, store undiluted between 2°C and 8°C.
WB - Quality tested
ChIP - Verified
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. For ChIP applications, the suggested dilution is 1:50-1:100 by volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Product Citations
AB_2562201 (BioLegend Cat. No. 655701)
AB_2562202 (BioLegend Cat. No. 655702)
- Human IRF-3 has 427 amino acids with predicted Mw 47.2kD and observed Mw 52kD. Contains an IRF tryptophan pentad repeat responsible for DNA binding. Mouse Irf-3 has 419 amino acids with predicted MW 46.9kD and observed Mw 45kD.
Inactive IRF-3 continuously transits between the cytoplasm and nucleus. Serine/threonine phosphorylation or IRF-3 results in the structural changes which allow IRF-3 activation. The activated IRF3 translocates to the nucleus, where it forms a complex with CREB binding protein and activates the transcription of interferons, interferon stimulated genes, and other antiviral response genes.
- IRF-3 is a constitutively expressed, latent transcription factor that plays a pivotal role in type I IFN response.
- CREB binding protein, IRF-7, TICAM-1, TICAM-2, non-canonical IKB kinases TBK1 and IKKε.
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Signal Transduction, Transcription Factors
- Molecular Family
- Nuclear Markers
- Antigen References
1. Lin R, et al. 1999. Mol. Cell Biol. 19:959.
2. Hiscott J, et al. 2007. J. Biol. Chem. 282:15325.
3. Molle C, et al. 2010. J. Immunol. 184:1784.
- Gene ID
- 3661 View all products for this Gene ID
- View information about IRF3 on UniProt.org
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