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Purified anti-IRF2 Antibody

Pricing & Availability
Clone
13B2A38 (See other available formats)
Regulatory Status
RUO
Other Names
Interferon regulatory factor 2 (IRF-2)
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
13B2A38_PURE_IRF2_Antibody_1_092518
Total cell lysates (15 µg total protein) from NTERA-2, HeLa, HEL, and Jurkat cells were resolved by 4-20% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 0.1 µg/mL (1:5000 dilution) of Purified anti-IRF2 Antibody, clone 13B2A38, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse-IgG Antibody (Cat. No. 405306). Direct-Blot™ HRP anti-β-Actin Antibody (Cat. No. 664804) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular Weight marker. Predicted expression data was obtained from Human Protein Atlas. Cell lysates were loaded in order of increasing IRF2 expression levels (source: Human Protein Atlas).
  • 13B2A38_PURE_IRF2_Antibody_1_092518
    Total cell lysates (15 µg total protein) from NTERA-2, HeLa, HEL, and Jurkat cells were resolved by 4-20% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 0.1 µg/mL (1:5000 dilution) of Purified anti-IRF2 Antibody, clone 13B2A38, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse-IgG Antibody (Cat. No. 405306). Direct-Blot™ HRP anti-β-Actin Antibody (Cat. No. 664804) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular Weight marker. Predicted expression data was obtained from Human Protein Atlas. Cell lysates were loaded in order of increasing IRF2 expression levels (source: Human Protein Atlas).
  • 13B2A38_PURE_IRF2_Antibody_2_092518
    Total cell lysates (15 µg total protein) from Jurkat and EL4 cells were resolved by 4-20% Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 0.1 µg/mL (1:5000 dilution) of Purified anti-IRF2 Antibody, clone 13B2A38, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse-IgG Antibody (Cat. No. 405306). Direct-Blot™ HRP anti-β-GAPDH Antibody (Cat. No. 607903) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular Weight marker. Predicted expression data was obtained from Human Protein Atlas. Cell lysates were loaded in order of increasing IRF2 expression levels (source: Human Protein Atlas).
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657502 100 µg £197
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Description

IRF2 is a member of the IRF family of transcription factors, mainly functioning as a transcriptional repressor. It binds to the interferon-sensitive response elements (ISREs) and competes for binding sites with the other IRF transcription factors, such as IRF1 and IRF9. Mice deficient in IRF2 spontaneously develop skin inflammation due to excessive activation of CD8+ T lymphocytes. Further, the severely reduced number of mature NK cells in IRF2-deficient mice suggests that IRF2 is required for normal NK cell development. IRF2 contains a latent activation domain that can act as a transcriptional activator and has been revealed to positively regulate Histone H4 and VCAM-1 gene expression.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial human IRF2 recombinant protein (151-349 a.a.)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

During PD testing for western blot application, this clone cross-reacted with a ~50 kDa protein of unknown origin when used at concentrations exceeding 0.1 µg/mL.

Product Citations
  1. Orzalli MH, et al. 2018. Mol Cell. 71:825. PubMed
RRID
AB_2562588 (BioLegend Cat. No. 657502)

Antigen Details

Structure
349 amino acids, predicted molecular weight of 39 kD; contains a IRF tryptophan pentad repeat responsible for DNA binding
Distribution

Nucleus

Function
Transcription factor, represses transcriptional activation of type I IFN and IFN-inducible genes
Interaction
Interacts with CREBBP, BRD7, IRF2BP1 and IRF2BP2
Cell Type
B cells, Dendritic cells
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Immunology, Innate Immunity, Transcription Factors
Antigen References

1. Hayashi H, et al. 2011. Proc. Natl. Acad. Sci. USA. 108:18766.
2. Hida S, et al. 2005. Blood. 106:2011.
3. Taki S, et al. 2005. J. Immunol. 174:6005.
4. Chung MC, et al. 2004. J. Biol. Chem. 279:56042.
5. Honda K, et al. 2004. Proc. Natl. Acad. Sci. USA. 101:2416.
6. Jesse TL, et al. 1998. J. Cell Biol. 140:1265.

Gene ID
3660 View all products for this Gene ID
UniProt
View information about IRF2 on UniProt.org
Go To Top Version: 6    Revision Date: 09/12/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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