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Purified anti-IL-17F Antibody (Previously Covance catalog# MMS-5118)

Pricing & Availability
Clone
6B9.H8 (See other available formats)
Regulatory Status
RUO
Other Names
Interleukin-17F, cytokine ML-1, mutant IL-17F
Previously
Covance Catalog# MMS-5118
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
6B9H8_Purified_IL-17F_Antibody_WB_122018_updated.png
Western blot of purified anti-IL-17F antibody (clone 6B9.H8). Lane 1, 3: Molecular weight marker; Lane 2: 20 µg of human colon lysate; Lane 3: 0.5 µg of recombinant human IL-17F protein. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • 6B9H8_Purified_IL-17F_Antibody_WB_122018_updated.png
    Western blot of purified anti-IL-17F antibody (clone 6B9.H8). Lane 1, 3: Molecular weight marker; Lane 2: 20 µg of human colon lysate; Lane 3: 0.5 µg of recombinant human IL-17F protein. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
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830201 100 µL £189
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Description

Interleukin 17F (IL-17F) is a 37 kD IL-17 family member.  The IL-17 family consists of six members including IL-17 (also called IL-17A), IL-17B, IL-17C, IL-17D, IL-17E (also called IL-25), and IL-17F.  IL-17F shares the strongest similarity to IL-17A and forms a homodimer or heterodimer with IL-17A. It is produced by Th17 cells, mast cells, basophils, and epithelial cells.  IL-17F is an important regulator of inflammatory responses.  It is involved in host defense against mucoepithelial infection by Staphylococcus aureus and Citrobacter rodentium.  Overexpression of the IL-17F gene in the airways of mice is associated with airway neutrophilia, the induction of many cytokines, an increase in airway hyperreactivity, and mucus hypersecretion. IL-17F is also involved in cancer immunity and autoimmune responses. IL-17F, like IL-17A, depends on IL-17R for its signaling in vitro and in vivo.  P38 MAPK, ERK1/2, Act1 (NF-kB activator protein 1), and TRAF6 are involved in IL-17F signaling.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against a peptide sequence conjugated to KLH corresponding to amino acids 107-120 of human IL-17F protein.
Formulation
Phosphate-buffered solution.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is effective in immunoblotting (WB).

RRID
AB_2564936 (BioLegend Cat. No. 830201)

Antigen Details

Structure
Expected MW: 15 kD
Biology Area
Cell Biology, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References

1. Dong C. 2008. Immunol. Rev. 226:80.
2. Kolls JK, et al. 2004. Immunity 21:467.
3. Aggarwal S, et al. 2002. J. Leukoc. Biol. 71:1.
4. Yang XO, et al. 2008. J. Exp. Med. 205:1063.
5. Ishigame H, et al. 2009. Immunity 30:108.
6. Hu Y, et al. 2010. J. Immunol. 184:4307.

Gene ID
112744 View all products for this Gene ID
UniProt
View information about IL-17F on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 06/23/2016

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This data display is provided for general comparisons between formats.
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