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Purified anti-human TNF-α Antibody

Pricing & Availability
Clone
MAb1 (See other available formats)
Regulatory Status
RUO
Other Names
Tumor necrosis factor-α, Cachectin, Necrosin, Macrophage cytotoxic factor (MCF), Differentiation inducing factor (DIF), TNFSF2
Isotype
Mouse IgG1, κ
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Product Citations
publications
HumanTNF-alpha_061604
  • HumanTNF-alpha_061604
Cat # Size Price Quantity Check Availability Save
502801 50 µg £57
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502802 500 µg £161
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Description

TNF-α is secreted by macrophages, monocytes, neutrophils, T-cells, and NK-cells. Many transformed cell lines also secrete TNF-α. Monomeric human TNF-α is a 157 amino acid protein (non-glycosylated) with a reported molecular weight of 17 kD. TNF-α forms multimeric complexes; stable trimers are most common in solution. A 26 kD membrane form of TNF-α has also been described. TNF-α binding to surface receptors elicits a wide array of biologic activities including: cytolysis and cytostasis of many tumor cell lines in vitro, hemorraghic necrosis of tumors in vivo, increased fibroblast proliferation, and enhanced chemotaxis and phagocytosis in neutrophils. The MAb1 antibody can neutralize the bioactivity of natural or recombinant TNF-α.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
E. coli-expressed, recombinant human TNF-α
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ELISA Capture - Quality tested
Neut, WB - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 0.25-1 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of TNF-α recombinant protein ranging from 500 to 4 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA or ELISPOT Capture: The purified MAb1 antibody is useful in neutralization2 and as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated MAb11 antibody (Cat. No. 502904/502914) as the detecting antibody. The Ultra-LEAF™ purified antibody is suggested for ELISPOT capture.

Application References

(PubMed link indicates BioLegend citation)
  1. Rathjen, D., et al. 1991. Mol. Immunol. 28:79.
  2. Verschoor CP, et al. 2015. Mol. Immunol. 65:148. (Neut) Pubmed
  3. Wyant, T. L., et al. 1999. Infect. Immun. 67:1338.
  4. Nichols, J. E., et al. 2001. J. Virol. 73:5921.
  5. Ivanov,VN.,et al. 1999. J. Biol Chem. 274:14079. PubMed
  6. Varadarajan N, et al. 2012. PNAS. 109:3885. PubMed.
Product Citations
  1. Varadarajan N, et al. 2012. Proc Natl Acad Sci U S A. 109:3885. PubMed
  2. Rodriguez-Salazar CA, et al. 2022. Viruses. 14:. PubMed
  3. Schwaderer J, et al. 2020. Cell Death Dis. 11:154. PubMed
  4. Li M, et al. 2022. J Biol Chem. 298:101543. PubMed
  5. Tao S, et al. 2020. Methods Mol Biol. 2108:181. PubMed
  6. Hammer Q, et al. 2018. Front Immunol. 1.705555556. PubMed
  7. Han G, et al. 2022. Ann Transl Med. 10:1134. PubMed
  8. Han X, et al. 2020. Mol Carcinog. 59:1088. PubMed
RRID
AB_315249 (BioLegend Cat. No. 502801)
AB_315250 (BioLegend Cat. No. 502802)

Antigen Details

Structure
TNF superfamily; dimer/trimer; 52 kD (Mammalian)
Bioactivity
Paracrine/endocrine mediator of inflammatory and immune functions; selectively cytotoxic for transformed cells; chemoattractant
Cell Sources
Activated monocytes, neutrophils, macrophages, T cells, B cells, NK cells, LAK cells
Cell Targets
Monocytes, neutrophils, macrophages, T cells, fibroblasts, endothelial cells, osteoclasts, adipocytes, astroglia, microglia
Receptors
TNFRSF1A (TNF-R1, CD120a, TNFR-p60 Type β, p55); TNFRSF1B (TNF-R2, CD120b, TNFR-p80 Type A, p75)
Cell Type
Tregs
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Beutler, B., et al. 1988. Annu. Rev. Biochem. 57:505.
3. Beutler, B., et al. 1989. Annu. Rev. Immunol. 7:625.
4. Tracey, K., et al. 1993. Crit. Care Med. 21:S415.

Regulation
Type II integral membrane protein processed by TACE for secretion; upregulated by interferons, IL-2, GM-CSF, substance P, bradykinin, PAF, immune complexes, cyclooxygenase; downregulated by IL-6, TGF-β, vitamin D3, prostaglandin E2, PAF antagonists,
Gene ID
7124 View all products for this Gene ID
UniProt
View information about TNF-alpha on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All TNF-α Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-human TNF-α MAb1 ELISA Capture,Neut,WB
Ultra-LEAF™ Purified anti-human TNF-α MAb1 ELISA Capture,ELISPOT Capture,Neut,WB
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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  • Purified anti-human TNF-α

    HumanTNF-alpha_061604

  • Ultra-LEAF™ Purified anti-human TNF-α
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