Purified anti-human T-bet Recombinant Antibody

Pricing & Availability
Clone
QA18A24 (See other available formats)
Regulatory Status
RUO
Other Names
T-box expressed in T cells, T box 21, TBLYM
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
QA18A24_-PURE_-T-bet_Antibody_2_121321
Human peripheral blood mononuclear cells (PBMC) were stained with anti-human CD3 APC. Cells were fixed and permeabilized with True-Nuclear™ Transcription Factor Buffer Set, and were stained with purified anti-human T-bet recombinant (clone QA18A24) (left) or purified mouse IgG1, κ isotype control (right), followed by anti-mouse IgG1 PE.
  • QA18A24_-PURE_-T-bet_Antibody_2_121321
    Human peripheral blood mononuclear cells (PBMC) were stained with anti-human CD3 APC. Cells were fixed and permeabilized with True-Nuclear™ Transcription Factor Buffer Set, and were stained with purified anti-human T-bet recombinant (clone QA18A24) (left) or purified mouse IgG1, κ isotype control (right), followed by anti-mouse IgG1 PE.
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365902 100 µg £150
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Description

T-bet, also known as T-box transcription factor T-bet, is considered to be a "master regulator" of Th1 lymphoid development controlling the production of the cytokine IFN-γ. T-bet is widely expressed in hematopoietic cells including stem cells, NK cells, B cells, and T cells. T-bet is critical for the control of microbial pathogens, and knockout animals show multiple physiologic and inflammatory features characteristic of asthma. T-bet expression is optimally observed after IL-12 stimulation and can be suppressed by addition of the Th2 cytokine IL-4 or neutralization of IL-12.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Recombinant
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone QA18A24 can completely block the binding of clone 4B10 on target cells.

RRID
AB_2904398 (BioLegend Cat. No. 365902)

Antigen Details

Structure
T-box transcription factor, approximately 58 kD.
Distribution

Nuclear; expressed in T cells, hematopoietic stem cells, NK cells, B cells, lung, spleen.

Function
Th1-specific T-box transcription factor controlling expression of the hallmark Th1 cytokine, interferon gamma (IFN-γ). T-bet expression is critical for the control of microbial pathogens.
Interaction
Human
Cell Type
B cells, Hematopoietic stem and progenitors, NK cells, T cells, Tregs
Biology Area
Cell Biology, Immunology, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References
  1. Szabo SJ, et al. 2000. Cell. 100:655-69.
  2. Szabo SJ, et al. 2002. Science. 295:338-42.
  3. Finotto S, et al. 2002. Science. 295:336-8.
  4. Mullen AC, et al. 2001. Science. 292:1907-10.

 

Gene ID
30009 View all products for this Gene ID
UniProt
View information about T-bet on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 12/13/2021

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Purified anti-human T-bet Recombinant Antibody

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