Purified anti-human Ki-67 Antibody

Pricing & Availability
Clone
W17211A (See other available formats)
Other Names
MKI67, Proliferation Marker Protein Ki-67, Antigen Ki67
Isotype
Rat IgG2a, κ
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Product Citations
publications
W17211A_PURE_Ki-67_Antibody_111519.png
Human paraffin-embedded intestine tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Citrate-Buffered 1X pH 6.0 at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 twice for five minutes, permerilized with 0.5% Triton X-100 for 10 minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of purified anti-human Ki-67 (clone W17211A) antibody overnight at 4°C. On the next day, tissue was incubate with Alexa Fluor® 594 goat anti-rat IgG (clone poly4054) antibody (red). Nuclei were counterstained with DAPI (blue). The image was scanned with a 10X objective and stitched with MetaMorph® software.
  • W17211A_PURE_Ki-67_Antibody_111519.png
    Human paraffin-embedded intestine tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Citrate-Buffered 1X pH 6.0 at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 twice for five minutes, permerilized with 0.5% Triton X-100 for 10 minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of purified anti-human Ki-67 (clone W17211A) antibody overnight at 4°C. On the next day, tissue was incubate with Alexa Fluor® 594 goat anti-rat IgG (clone poly4054) antibody (red). Nuclei were counterstained with DAPI (blue). The image was scanned with a 10X objective and stitched with MetaMorph® software.
  • W17211A_PURE_Ki-67_Antibody_2_111519.png
    Human paraffin-embedded tonsil tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Citrate-Buffered 1X pH 6.0 at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 twice for five minutes, permerilized with 0.5% Triton X-100 for 10 minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of purified anti-human Ki-67 (clone W17211A) antibody overnight at 4°C. On the next day, tissue was incubate with Alexa Fluor® 594 goat anti-rat IgG (clone poly4054) antibody (red). Nuclei were counterstained with DAPI (blue). The image was scanned with a 10X objective and stitched with MetaMorph® software.
Cat # Size Price Quantity Avail. Save
398502 100 µg £79
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Description

Antigen Ki-67 is a nuclear protein expressed as two isoforms with molecular weights of 395 and 345 kD. Both isoforms contain one forkhead-associated domain and 16 concatenated "Ki-67 repeats," each containing the epitope recognized by the mAb Ki-67. The antigen Ki-67 interacts with Hklp2, hNIFK, and chromobox protein homolog 1, 3, and 5. Ki-67 is required for cell proliferation and its expression is restricted to the phases G1, S, G2, and M of the cell cycle. This characteristic makes Ki-67 an excellent marker for proliferating cells and is commonly used as one of the prognostic factors in cancer studies. Ki-67 has also been used to study myocyte proliferation after myocardial infarction as well as lymphocyte proliferation during infection, and has been used in neurons of patients with different neuropathologies.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Human Ki-67 recombinant protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Antigen Details

Structure
Two isoforms with molecular weights of 395 and 345 kD, one forkhead-associated domain, 16 concatenated Ki-67 repeats, located in nucleus
Distribution

Expressed in the phases G1, S, G2, and M of the cell cycle

Function
Required for cell proliferation
Interaction
Chromobox protein homolog 1, 3 and 5, Hklp2, and hNIFK
Biology Area
Cell Biology, Cell Cycle/DNA Replication, DNA Repair/Replication
Molecular Family
Nuclear Markers
Antigen References
  1. Byeon IJ, et al. 2005. Nat. Struct. Mol. Biol. 12:987.
  2. Yerushalmi R, et al. 2010. Lancet. Oncol. 11:174.
  3. Drazen JM. et al. 2001. N. Engl. J. Med. 344:1750.
  4. Sachsenberg N, et al. 1998. J. Exp. Med. 187:1295.
  5. Nagy Z, et al. 1997. Acta. Neuropathol. 93:294.
Gene ID
4288 View all products for this Gene ID
UniProt
View information about Ki-67 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 11/15/2019

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