Purified anti-human IL-16 Antibody

Pricing & Availability
14.1 (See other available formats)
Regulatory Status
Other Names
Mouse IgG2a
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Product Citations
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519102 100 µg £117
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Interleukin-16 is a proinflammatory cytokine synthesized as a 68 kD precursor molecule (pro-IL-16). After cleavage of pro-IL-16 by caspase-3, IL-16 is released as a 12 kD protein, but its biologic activities are exerted by a tetramer. IL-16 is a chemoattractant for CD4+ T cells, monocytes, and eosinophils. On T cells, IL-16 upregulates CD25 and class II expression, and also inhibits HIV-1 replication in vitro by repressing the transcription of the HIV-1 long terminal repeat. IL-16 is expressed by lymphocytes, macrophages, eosinophils, mast cells, epithelial cells, and fibroblasts. CD4 and CD9 are the receptors of IL-16.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Reported Reactivity
Antibody Type
Host Species
Peptide corresponding to amino acids 615 to 630 (RRKSLQSKETTAAGDS) of IL-16
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
The antibody was purified by affinity chromatography.
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.

ICFC - Quality tested
ELISA, IHC-F, Block - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: ELISA2, inhibition of T cell chemotaxis2 and immunohistochemical staining of frozen lymph node sections3.

Application References

(PubMed link indicates BioLegend citation)
  1. Cruikshank WW, et al. 1994. P. Natl Acad Sci USA 91:5109. (ICFC)
  2. Keane J, et al. 1998. J. Immunol. 160:5945. (Block, ELISA)
  3. Chupp GL, et al. 1998. J. Immunol. 161:3114. (Block, IHC)
  4. Stoitzner P, et al. 2001. J. Invest. Dermatol. 116:641. (ICFC)
  5. Ren F, et al. 2005. J. Immunol. 174:2738. (ICFC)
Product Citations
  1. Noman M, et al. 2015. Cancer Res . 75: 3771-3787. PubMed
AB_11204433 (BioLegend Cat. No. 519102)

Antigen Details

12 kD protein with a PDZ domain, product of the cleavage of pro-IL-16 by caspase-3, forms tetramers.

Lymphocytes, macrophages, eosinophils, mast cells, epithelial cells, and fibroblasts.

Chemoattractant for CD4+ T cells, monocytes, and eosinophils. Upregulates CD25 and class II expression on T cells. Inhibits HIV-1 replication.
Cell Type
Eosinophils, Epithelial cells, Fibroblasts, Lymphocytes, Macrophages, Mast cells, Tregs
Biology Area
Immunology, Innate Immunity
Molecular Family
Antigen References

1. Bellon M, et al. 2010. Blood 115:2441.
2. Rofani C, et al. 2009. Stem Cells Dev. 18:671.
3. Cruikshank W and Little F. 2008. Crit. Rev. Immunol. 28:467.
4. Gu Y, et al. 2008. J. Immunol. 181:4418.
5. McFadden C, et al. 2007. J. Immunol. 179:6439.
6. Qi JC, et al. 2006. Blood 107:135.

Gene ID
3603 View all products for this Gene ID
View information about IL-16 on UniProt.org

Related FAQs

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Go To Top Version: 4    Revision Date: 08/11/2022

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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