- 7B12 (See other available formats)
- Regulatory Status
- Other Names
- Interleukin-12, Cytotoxic lymphocyte maturation factor (CLMF), Natural killer cell stimulatory factor (NKSF), CTL maturation factor (TcMF), T-cell stimulating factor (TSF)
- Rat IgG1
- Ave. Rating
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- Product Citations
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Bioactive IL-12 is a potent regulator of cell-mediated immune responses and key mediator of Th1 cell development. Bioactive IL-12 is secreted by activated B lymphocytes, dendritic cells and macrophages as a 70 kD heterodimeric glycoprotein comprised of disulfide-bonded 35 kD and 40 kD subunits. The disulfide-linked p40 homodimer can bind to IL-12 receptors and antagonize the activities of bioactive IL-12. The 7B12 antibody reacts with human IL-12 p70 heterodimer, but shows no reactivity with IL-12 p35 or p40 monomer. The 7B12 has high affinity to human IL-12 p70 heterodimer.Product Details
- Human, p70 heterodimer
- Antibody Type
- Host Species
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Recommended Usage
Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 1-4 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of IL-12 p70 recombinant protein ranging from 1000 to 8 pg/ml are recommended for each ELISA plate. For use as an ELISPOT capture antibody, a concentration range of 2-6 µg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
ELISA or ELISPOT capture: The purified 7B12 antibody is useful as a capture antibody for an IL-12 p70 specific sandwich ELISA or ELISPOT, when used in conjunction with Biotin anti-human IL-12/IL-23 p40 antibody (Cat. No. 508802/508805) as the detection antibody. The Ultra-LEAF™ Purified antibody is suggested for ELISPOT capture.
Note: For testing human IL-12/IL-23 p40 (monomer, dimer) in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. No. 430701) are specially developed and recommended. For testing human IL-12 p70 in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. No. 431701) are specially developed and recommended.
(PubMed link indicates BioLegend citation)
- Shimomura Y, et al. 2008. J. Exp. Med. 205:1343. PubMed
- Product Citations
AB_2304113 (BioLegend Cat. No. 511001)
AB_2211913 (BioLegend Cat. No. 511002)
- Cytokine; monomer, dimer (with p35 or p40)
- Induces IFN-γ, TNF-α production in T and NK cells; costimulation of PBL proliferation; proliferation/differentiation of TH1 T lymphocytes
- Cell Sources
- Dendritic cells, monocytes/macrophages, B cells, T cells
- Cell Targets
- T cells, NK cells, PBL
- IL-12Rβ1 binds p40; dimeric with IL-12Rβ2 binds p35
- Biology Area
- Molecular Family
- Antigen References
1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Quesniaux V. 1992. Research Immunol. 143:385.
3. Trinchieri G, et al. 1992 Prog. Growth Factor Res. 4:355.
4. Trinchieri G, et al. 1993 Immunol. Today. 14:335.
5. Oppmann B, et al. 2000 Immunity. 13:715.
6. Aggarwal S, et al. 2003 J. Biol. Chem.. 278:1910.
7. Parham C, et al. 2002 J. Immunol.. 168:5699.
8. Belladonna ML, et al. 2002 J. Immunol.. 168:5448.
9. Lankford CS, et al. 2003 J. Leukoc. Biol.. 73:49.
- Downregulated by IL-10; homodimeric p40 antagonistic to functional p70 heterodimer; p40 monomer has no function; p40 subunit in common with IL-23
- Gene ID
- 3592 View all products for this Gene ID 3593 View all products for this Gene ID
- View information about IL-12 p70 on UniProt.org
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