- IA6-2 (See other available formats)
- Regulatory Status
- Other Names
- Ig delta chain C region
- Mouse IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
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IgD, a member of the immunoglobulin (Ig) family, is expressed in naïve B cells. It has 3 Ig-like domains and exists in a transmembrane and a soluble form. In general, IgD is not secreted and usually its expression is lost after the Ig isotype switch. After antigen binding, IgD signals through the CD79a/CD79b (Igα/Igβ) heterodimer, resulting in the activation of the B cell.Product Details
- Verified Reactivity
- Antibody Type
- Host Species
- Human IgD
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.
- The antibody was purified by affinity chromatography.
- 1.0 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
FC - Quality tested
CyTOF® - Verified
- Recommended Usage
This product is suitable for use with the Maxpar® Metal Labeling Kits. For metal labeling using Maxpar® Ready antibodies, proceed directly to the step to Partially Reduce the Antibody by adding 100 µl of Maxpar® Ready antibody to 100 µl of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar® User Guide when conjugating Maxpar® Ready antibodies.
- Application Notes
Additional reported applications (for the relevant formats) include: immunohistochemical staining of paraformaldehyde fixed frozen sections4 and spatial biology (IBEX)5,6.
- Additional Product Notes
Maxpar® is a registered trademark of Standard BioTools Inc.
(PubMed link indicates BioLegend citation)
- Chen K, et al. 2009. Nat. Immunol. 10:889.
- Lee CH, et al. 2005. J. Exp. Med. 203:63.
- Sutter JA, et al. 2008. Clin. Immunol. 126:282.
- Li H and Pauza CD. 2015. Eur. J. Immunol. 45:298. (IHC)
- Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
- Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
- Product Citations
AB_2563775 (BioLegend Cat. No. 348235)
- Exists in a transmembranal and a soluble form
Naïve B cells
- Antigen binding, B cell activation
- The CD79a/CD79b heterodimer
- Cell Type
- B cells
- Biology Area
- Antigen References
1. Geisberger R, et al. 2006. Immunology 118:429.
2. Weller S, et al. 2005. Eur. J. Immunol. 35:2789.
3. Brandtzaeg P and Johansen FE. 2005. Immunol. Rev. 206:32.
- Gene ID
- 3495 View all products for this Gene ID
- View information about IgD on UniProt.org
- Can I obtain CyTOF data related to your Maxpar® Ready antibody clones?
We do not test our antibodies by mass cytometry or on a CyTOF machine in-house. The data displayed on our website is provided by Fluidigm®. Please contact Fluidigm® directly for additional data and further details.
- Can I use Maxpar® Ready format clones for flow cytometry staining?
We have not tested the Maxpar® Ready antibodies formulated in solution containing EDTA for flow cytometry staining. While it is likely that this will work in majority of the situations, it is best to use the non-EDTA formulated version of the same clone for flow cytometry testing. The presence of EDTA in some situations might negatively affect staining.
- I am having difficulty observing a signal after conjugating a metal tag to your Maxpar® antibody. Please help troubleshoot.
We only supply the antibody and not test that in house. Please contact Fluidigm® directly for troubleshooting advice: http://techsupport.fluidigm.com/
- Is there a difference between buffer formulations related to Maxpar® Ready and purified format antibodies?
The Maxpar® Ready format antibody clones are formulated in Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA. The regular purified format clones are formulated in solution that does not contain any EDTA. Both formulations are however without any extra carrier proteins.
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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PerCP anti-human IgD
Brilliant Violet 605™ anti-human IgD
Alexa Fluor® 700 anti-human IgD
Purified anti-human IgD
PE anti-human IgD
Biotin anti-human IgD
FITC anti-human IgD
PerCP/Cyanine5.5 anti-human IgD
PE/Cyanine7 anti-human IgD
Alexa Fluor® 488 anti-human IgD
APC/Cyanine7 anti-human IgD
Brilliant Violet 510™ anti-human IgD
APC anti-human IgD
Pacific Blue™ anti-human IgD
Brilliant Violet 421™ anti-human IgD
Purified anti-human IgD (Maxpar® Ready)
PE/Dazzle™ 594 anti-human IgD
APC/Fire™ 750 anti-human IgD
Brilliant Violet 785™ anti-human IgD
TotalSeq™-A0384 anti-human IgD
TotalSeq™-C0384 anti-human IgD
TotalSeq™-B0384 anti-human IgD
PE/Cyanine5 anti-human IgD
TotalSeq™-D0384 anti-human IgD
Spark UV™ 387 anti-human IgD