Purified anti-human IFN-β Antibody

Pricing & Availability
Clone
IFNb/A1 (See other available formats)
Other Names
Interferon-β, Fibroblast interferon (Fi-IFN, F-IFN), Type-1 interferon, acid-stable interferon, R1-GI factor, IFN-b, IFN-beta
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
IFNbA1_PURE_IFN-Beta_Antibody_1_WB_101716
Recombinant human IFN-β (50 ng, lane 1) and recombinant mouse IFN- β (50 ng, lane 2) were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with purified anti-human IFN-β (clone IFNb/A1) antibody. Proteins were visualized using a Goat anti-mouse-IgG secondary antibody conjugated to HRP and chemiluminescence detection.
  • IFNbA1_PURE_IFN-Beta_Antibody_1_WB_101716
    Recombinant human IFN-β (50 ng, lane 1) and recombinant mouse IFN- β (50 ng, lane 2) were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with purified anti-human IFN-β (clone IFNb/A1) antibody. Proteins were visualized using a Goat anti-mouse-IgG secondary antibody conjugated to HRP and chemiluminescence detection.
Cat # Size Price Quantity Avail. Save
514002 200 µg £168
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Description

The IFNb/A1 clone recognizes natural human IFN-β and recombinants β1a and β1b.It is capable of neutralizing IFN-β.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, epitope: Hu IFN-β site I, core peptide amino acid residues 32-56, AB loop (AB2, AB3)
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant HuIFN-β1b
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ELISA, Neut - Reported in the literature

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 µg antibody per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Neutralization1: The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of human IFN-β bioactivity (Cat. No. 514003).

Additional reported applications (for the relevant formats) include: ELISA1.

Application References

(PubMed link indicates BioLegend citation)
  1. Redlich PN and Grossberg SE, et al. 1989. J. Immunol. 143:1887. (ELISA, Neut)
  2. Redlich PN, et al. 1991. P. Natl. Acad. Sci. USA 88:4040.
  3. Runkel L, et al. 2001. IFN & Cytokine Res. 21:931.
RRID
AB_2122765 (BioLegend Cat. No. 514002)

Antigen Details

Structure
Interferon; contains 187 amino acids with predicted molecular weight 22 kD.
Bioactivity
Non-specific humoral immune responses and immune responses against viral infections, increases expression of MHC class I antigens, blocks expression of MHC class II antigens stimulated by IFN-γ
Cell Sources
Fibroblasts, epithelial cells
Cell Targets
Broad, includes NK cells, T suppressor cells, activated monocytes
Receptors
IFNα/βR (IFNAR-1, IFNAR-2)
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Signal Transduction
Molecular Family
Cytokines/Chemokines
Antigen References

1. Nagarajan U, 2011. Crit. Rev. Immunol. 31:459.
2. Honda K, et al. 2005. Int. Immunol. 17:1367.

Regulation
Upregulated by viruses, double-stranded RNA, micro-organisms, TNF, IL-1
Gene ID
3456 View all products for this Gene ID
UniProt
View information about IFN-beta on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 5    Revision Date: 10/17/2016

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