- 3H4 (See other available formats)
- Regulatory Status
- Other Names
- Heparin-binding EGF-like growth factor, HB-EGF-like growth factor
- Mouse IgG1, κ
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- Product Citations
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Heparin-binding EGF-like growth factor (HB-EGF) is a member of the EGF family of growth factors. Human HB-EGF was initially identified as a protein of 22 kD secreted by macrophage-like U937 cells. It belongs to the EGF family of proteins that includes EGF, TGF-α, HB-EGF, epigen, epiregulin, betacellulin, neuroregulin, and tomoregulin. All the EGF family members are synthesized as type I membrane protein precursors, which can undergo proteolytic cleavage at the plasma membrane to release a mature soluble ectodomain. It has been suggested that various metalloproteinases participate in the shedding of HB-EGF, such as MMP-3, MMP-7, ADAM9, ADAM10, ADAM12, and ADAM17. The ectodomain shedding is stimulated by phorbol esters, calcium ionophore, lysophosphatidic acid, and IL-1β. In addition, nardilysin, a metalloendopeptidase of the M16 family, binds HB-EGF and enhances its shedding through activation of TACE (ADAM17). The membrane-anchored HB-EGF acts in cell-cell and cell-extracellular matrix interactions. In addition, the membrane HB-EGF is the receptor for diphtheria toxin that can be internalized and induce apoptotic death. HB-EGF plays a crucial role in cardiac valvulogenesis. Newborn knockout mice have malformed semilunar and atrioventricular heart valves and poorly differentiated lungs. HB-EGF is overexpressed in several types of cancer such as pancreatic carcinoma, ovarian cancer, and gastric carcinoma. HB-EGF is also involved in cancer metastasis and invasion of ovarian cancer, head and neck cancer, and thyroid carcinoma cells.Product Details
- Verified Reactivity
- Antibody Type
- Host Species
- Human HB-EGF, extracellular domain (recombinant)
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
FC - Quality tested
ICC, WB, IP - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
(PubMed link indicates BioLegend citation)
- Hamaoka M, et al. 2010. J Biochem. 148:55.
AB_2927862 (BioLegend Cat. No. 383702)
Monocytes, macrophages, T cells, neutrophils, eosinophils, myeloid leukemia blasts, myeloma cells, vascular smooth muscle cells, endothelial cells, and normal or neoplastic epithelial cells
- HB-EGF is associated with proliferation and differentiation of stromal cells, apoptosis, and morphogenesis. It participates in inflammation, wound healing, atherosclerotic plaque progression, and cardiac valvulogenesis. TNF-α and IL-1β induces HB-EGF in endothelial cells.
- Fibroblast, smooth muscle cells, monocytes/macrophages, endothelial cells, astrocytes, keratinocytes, normal and neoplastic epithelial cells
- EGFR (ErbB1) and ErbB4; also binds to heparan-sulphate proteoglycans
- Cell Type
- Epithelial cells, Macrophages, T cells
- Biology Area
- Angiogenesis, Cancer Biomarkers
- Molecular Family
- Growth Factors
- Antigen References
- Higashiyama S, et al. 1991. Science. 251:936.
- Hirata M, et al. 2001. Biochem. Biophys. Res. Commun. 283:915.
- Jackson LF, et al. 2003. EMBO J. 22:2704.
- Nishi E, et al. 2006. J. Biol. Chem. 281:31164.
- Yotsumoto F, et al. 2008. Biochem. Biophys. Res. Commun. 365:555.
- Ota I, et al. 2013. Oncol Rep. 30:1593.
- Gene ID
- 1839 View all products for this Gene ID
- View information about HB-EGF on UniProt.org
Related Pages & Pathways
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|Purified anti-human HB-EGF||3H4||FC,ICC,WB,IP|
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