Purified anti-human CD9 Antibody

Pricing & Availability
Clone
HI9a (See other available formats)
Regulatory Status
RUO
Workshop
V P018
Other Names
Tetraspanin, MRP-1, DRAP-24
Isotype
Mouse IgG1, κ
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Product Citations
publications
HI9a
Human platelets stained with purified HI9a, then detected with anti-mouse IgGs FITC
  • HI9a
    Human platelets stained with purified HI9a, then detected with anti-mouse IgGs FITC
  • HI9a_PURE_CD9_Antibody_ICC_011921
    BT474 breast cancer cell line was stained with anti-human CD9, detected with anti-mouse DyLight™ 649, and nuclear counterstained with DAPI. Images were acquired with a TE300 fluorescence microscope with a 20x objective. Data provided by: Er Liu and John Nolan, La Jolla Bioengineering Institute
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312102 100 µg £77
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Description

CD9 is a 24 kD type III transmembrane protein also known as tetraspanin, MRP-1 and DRAP-24. It is a member of the tetraspan family (spanning the membrane four times) found on platelets, B cell progenitors, activated lymphocytes, granulocytes, endothelial cells and epithelial cells. CD9 induces adhesion, platelet aggregation, and B cell development. CD9 has been shown to associate with CD63, CD81, CD82, and CD36 and to bind to β1 integrins.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon, Cow, Cynomolgus, Dog, Horse, Rabbit, Rhesus, Sheep
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
ICC - Verified

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, it is recommended to use at ≤ 0.5 µg per 106 cells in 100 µL volume or 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
Product Citations
  1. Ma Y, et al. 2021. Sci Rep. 11:13471. PubMed
  2. Zhang X, et al. 2020. J Extracell Vesicles. 9:1791450. PubMed
  3. Chernyshev VS, et al. 2022. J Extracell Vesicles. 11:e12256. PubMed
  4. Temoche‐Diaz MM et al. 2019. Elife. 8 pii: e47544. PubMed
  5. Desjardins P, et al. 2022. Int J Mol Sci. 23:. PubMed
  6. Kobayashi Y, et al. 2021. Front Physiol. 12:693007. PubMed
  7. Valkov N, et al. 2021. Life Sci Alliance. 4:. PubMed
  8. McQuade A, et al. 2020. Nat Commun. 4.1875. PubMed
  9. Sayeed N, et al. 2022. PLoS One. 17:e0272511. PubMed
  10. Zabegina L, et al. 2021. Cells. 10:. PubMed
  11. Nakai W, et al. 2016. Sci Rep. 6: 33935. PubMed
  12. Olivia S Chao et al. 2017. Journal of cellular biochemistry. 118(12):4414-4424 . PubMed
  13. Lu-Culligan A, et al. 2021. Med. 2(5):591-610.e10. PubMed
  14. Araki Y, et al. 2021. Front Oncol. 11:667109. PubMed
  15. Lim K, et al. 2021. J Extracell Vesicles. 10:e12170. PubMed
  16. Fitzgerald W, et al. 2018. Am J Reprod Immunol. 80:e12860. PubMed
  17. Zheng W, et al. 2022. Nat Biomed Eng. 6:979. PubMed
  18. Kretschmann S, et al. 2019. J Clin Invest. 130:2952. PubMed
  19. Hasselmann J, et al. 2020. Neuron. 103(6):1016-1033. PubMed
  20. Dooley K, et al. 2021. Mol Ther. 29:1729. PubMed
  21. Bitirim CV, et al. 2022. Sci Rep. 12:5651. PubMed
  22. Layton TB, et al. 2020. Nat Commun. 2.380555556. PubMed
  23. Bachurski D, et al. 2019. J Extracell Vesicles. 8:1596016. PubMed
  24. Andriessen A, et al. 2022. Vet Comp Oncol. 20:381. PubMed
  25. Claes C, et al. 2021. Mol Neurodegener. 16:50. PubMed
  26. Gunasekaran M, et al. 2020. J Heart Lung Transplant. 39:379. PubMed
  27. Dobrowolski M, et al. 2020. Development. . PubMed
  28. Lobastova L, et al. 2021. Frontiers in Cell and Developmental Biology. 9:698503. PubMed
  29. Khan AA, et al. 2022. Bioconjug Chem. . PubMed
  30. Kawakami K, et al. 2021. Sci Rep. 11:15000. PubMed
  31. NULL, et al. 2022. Cell. 185:916. PubMed
  32. Fordjour FK, et al. 2022. J Biol Chem. :102394. PubMed
  33. Dobrowolski M, et al. 2020. Development. 147:00:00. PubMed
  34. Zhang X, et al. 2020. Int J Mol Sci. 21:00. PubMed
  35. Kennedy-Darling J, et al. 2021. Eur J Immunol. 51:1262. PubMed
  36. Yao PJ, et al. 2021. Biomedicines. 9:. PubMed
  37. Arab T, et al. 2021. J Extracell Vesicles. 10:e12079. PubMed
  38. Dörsam B, et al. 2018. Front Immunol. 7:e34961. PubMed
  39. Li H, et al. 2020. Haematologica. 105:1206. PubMed
  40. Wagner M, et al. 2020. Nat Commun. 1147:11. PubMed
  41. Klymiuk MC, et al. 2019. BMC Vet Res. 15:42. PubMed
RRID
AB_314907 (BioLegend Cat. No. 312102)

Antigen Details

Structure
Tetraspan family, type III transmembrane protein, 24 kD
Distribution

Platelets, B cell progenitors, activated lymphocytes, granulocytes, endothelial and epithelial cells

Function
Adhesion, platelet activation, B cell development
Ligand/Receptor
Associates with CD63, CD81, CD82 and CD36, binds PSG17
Cell Type
B cells, Embryonic Stem Cells, Endothelial cells, Epithelial cells, Granulocytes, Lymphocytes, Platelets
Biology Area
Immunology, Stem Cells
Molecular Family
CD Molecules
Antigen References

1. Miao WM, et al. 2001 Blood 97:1689.
2. Ellerman DA, et al. 2003 Mol. Biol Cell. (Epub ahead of print).
3. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.

Gene ID
928 View all products for this Gene ID
UniProt
View information about CD9 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 01/19/2021

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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