- NKI-SAM-1 (See other available formats)
- Other Names
- α5 integrin, VLA-5 α chain, Integrin α5 chain, ITGA5
- Mouse IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
|Cat #||Size||Price||Quantity Avail.||Save|
CD49e is a type I integral membrane glycoprotein, known as α5 integrin, VLA-5 α chain. It forms noncovalent heterodimer with integrin β1 (CD29). CD49e contains two disulfide-linked subunits of 135 kD and 24 kD, and is mainly expressed on thymocytes, activated lymphocytes, endothelial cells, osteoblasts, melanoma, and some myeloid leukemia cells. CD49e/CD29 complex binds to fibronection and neural adhesion molecule L1. It mediates monocytes migration, involves in the regulation of cells survival and apoptosis, and co-stimulates T cell activation.Product Details
- Human, African Green, Baboon, Cynomolgus, Rhesus
- Antibody Type
- Host Species
- U937 cells
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
FC - Quality tested
IP, ELISA - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported (for the relevant formats) applications include: immunohistochemical staining of acetone-fixed frozen sections, immunoprecipitation, and inhibition of a5 integrin mediated cell adhesion2. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 328004).
(PubMed link indicates BioLegend citation)
- Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
- Orecchia A, et al. 2003. J. Cell Sci. 116:3479. (Block)
- Product Citations
AB_893363 (BioLegend Cat. No. 328002)
- Integrin family, associates with integrin ß1 (CD29), 135 kD and 25 kD in reduced condition, 155 kD (unreduced)
Thymocytes, activated lymphocytes, endothelial cells, osteoblasts, melanoma, some myeloid leukemia cells
- Adhesion, regulation of cell survival and apoptosis
- Fibronectin, neural adhesion molecule L1
- Cell Type
- Endothelial cells, Leukemia, Lymphocytes, Mesenchymal Stem Cells, Osteoclasts, Thymocytes
- Biology Area
- Cell Adhesion, Cell Biology, Immunology, Stem Cells
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
1.Tran Van Nhieu GT and Isberg RR. 1991. J. Biol. Chem. 266:24367.
2. Isaji T, et al. 2006.J. Biol. Chem. 281:33258.
- Gene ID
- 3678 View all products for this Gene ID
- View information about CD49e on UniProt.org
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.