Purified anti-human CD4 Antibody

Pricing & Availability
Clone
OKT4 (See other available formats)
Regulatory Status
RUO
Workshop
HCDM listed
Other Names
T4
Isotype
Mouse IgG2b, κ
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Product Citations
publications
1_OKT4_Purified_060507
Human peripheral blood lymphocytes stained with purified OKT4, followed by anti-mouse IgGs FITC
  • 1_OKT4_Purified_060507
    Human peripheral blood lymphocytes stained with purified OKT4, followed by anti-mouse IgGs FITC
  • 2_OKT4_PURE_CD4_Antibody_IHC-F_090718
    Human frozen spleen tissue slices were fixed with 4% PFA for ten minutes and blocked with 5% FBS for 30 minutes. Then, the tissue was stained with 10 µg/mL of purified anti-human CD4 antibody (clone OKT4) overnight at 4°C. On the next day, tissue was incubated with Alexa Fluor® 594 Goat anti-mouse IgG (clone Poly4053, red). Nuclei were counter-stained with DAPI (blue). The image was scanned with a 10X objective and stitched with MetaMorph® software.
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317401 25 µg £10.00
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317402 100 µg £24.00
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Description

CD4, also known as T4, is a 55 kD single-chain type I transmembrane glycoprotein expressed on most thymocytes, a subset of T cells, and monocytes/macrophages. CD4, a member of the Ig superfamily, recognizes antigens associated with MHC class II molecules and participates in cell-cell interactions, thymic differentiation, and signal transduction. CD4 acts as a primary receptor for HIV, binding to HIV gp120. CD4 has also been shown to interact with IL-16. 

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Cross-Reactivity: Chimpanzee, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human peripheral T cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F - Verified

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. For immunohistochemistry, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The OKT4 antibody binds to the D3 domain of CD4 and does not block HIV binding. Additional reported applications (for the relevant formats) include: immunohistochemistry of frozen sections and blocking of T cell activation. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 317453 and 317454).

In a small subset of individuals, the OKT4 clone does not bind to CD4 due to polymorphisms in CD4.

Application References

(PubMed link indicates BioLegend citation)
  1. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  2. Reinherz EL, et al. 1979. Proc. Natl. Acad. Sci. 76:4061.
  3. Kmieciak M, et al. 2009. J. Transl. Med. 7:89. (FC) PubMed
  4. Cicin-Sain L, et al. 2010. J. Immunol. 184:6739. PubMed
  5. Rosenzweig M, et al. 2001. J. Med. Primatol. 30:36.
  6. Linder J, et al. 1987. Am. J. Pathol. 127:1.
  7. Boche D, et al. 1999. J. Neurovirol. 5:232. (IHC)
  8. Reinherz EL, et al. 1979. Proc. Natl. Acad. Sci. USA. 76:4061. (Immunogen)
Product Citations
  1. Martins M, et al. 2017. PLoS Pathog. 10.1371/journal.ppat.1006529. PubMed
  2. Santos R, et al. 2017. Nat Commun. . 10.1038/s41467-017-01760-5. PubMed
  3. Ma F, et al. 2017. Exp Cell Res.. 10.1016/j.yexcr.2017.10.011. PubMed
  4. Fletcher–Jones A, et al. 2019. Elife. 8:e44252. PubMed
  5. Martins MA, et al. 2017. AIDS Res Hum Retroviruses. 33:843. PubMed
  6. Alsahafi N, et al. 2017. J Virol. 91:e00109. PubMed
  7. Volchenkov R, et al. 2017. Front Immunol. 0.818055556. PubMed
  8. Cardoso V, et al. 2017. Nature. 549:277. PubMed
  9. Horn LA, et al. 2017. Oncotarget. 8:57964. PubMed
  10. Matos TR, et al. 2017. J Clin Invest. 127:4031. PubMed
  11. Prévost J, et al. 2018. Virology. 515:38:00. PubMed
  12. Brinkmann CR, et al. 2018. mSphere. 3:e00616. PubMed
  13. Wang B, et al. 2018. Mol Ther Nucleic Acids. 0.548611111. PubMed
  14. Ardain A, et al. 2019. Nature. 570:528. PubMed
  15. Majri SS, et al. 2018. J Immunol. 200:110. PubMed
  16. Seyfarth J, et al. 2018. Mol Cell Pediatr. 5:2. PubMed
  17. Li Y, et al. 2019. Front Immunol. 0.460416667. PubMed
  18. Kong R, et al. 2019. Cell. 178:567. PubMed
  19. Ghosh S, et al. 2020. Cell Reports. 30(10):3434-3447. PubMed
  20. Oberg HH, et al. 2020. J Leukoc Biol. 107:1081. PubMed
  21. Gernoux G, et al. 2020. Mol Ther. 28:747. PubMed
  22. Lou F, et al. 2020. Immunity. 53(1):204-216.e10. PubMed
  23. Wastyk HC, et al. 2021. Cell. 184(16):4137-4153.e14. PubMed
  24. Martin E, et al. 2020. JCI Insight. :5. PubMed
RRID
AB_571962 (BioLegend Cat. No. 317401)
AB_571963 (BioLegend Cat. No. 317402)

Antigen Details

Structure
Ig superfamily, type I transmembrane glycoprotein, 55 kD
Distribution

T cell subset, majority of thymocytes, monocytes/macrophages

Function
MHC class II co-receptor, lymphocyte adhesion, thymic differentiation, HIV receptor
Ligand/Receptor
MHC class II molecules, HIV gp120, IL-16
Cell Type
Macrophages, Monocytes, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Center D, et al. 1996. Immunol. Today 17:476.
2. Gaubin M, et al. 1996. Eur. J. Clin. Chem. Clin. Biochem. 34:723.

Gene ID
920 View all products for this Gene ID
UniProt
View information about CD4 on UniProt.org

Related FAQs

I am unable to see expression of T cell markers such as CD3 and CD4 post activation.
TCR-CD3 complexes on the T-lymphocyte surface are rapidly downregulated upon activation with peptide-MHC complex, superantigen or cross-linking with anti-TCR or anti-CD3 antibodies. PMA/Ionomycin treatment has been shown to downregulate surface CD4 expression. Receptor downregulation is a common biological phenomenon and so make sure that your stimulation treatment is not causing it in your sample type.
Go To Top Version: 4    Revision Date: 07/13/2015

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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