Purified anti-human CD32B/C Antibody

Pricing & Availability
Clone
S18005H (See other available formats)
Other Names
FCGR2B, FCgRIIB, FCGR2C, FCRIIC, IGFR2, CD32B, CD32C, FCG2, FCGR2, IGFR2, FcRII-c, CDW32
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
a-S18005H_PURE_CD32B-C_Antibody_1_082119.png
Human peripheral blood lymphocytes were stained with CD19 PE/Cyanine7 and CD32B/C (clone S18005H) Purified (left) or Mouse IgG1, κ Purified isotype control (right) followed by anti-mouse IgG1 PE
  • a-S18005H_PURE_CD32B-C_Antibody_1_082119.png
    Human peripheral blood lymphocytes were stained with CD19 PE/Cyanine7 and CD32B/C (clone S18005H) Purified (left) or Mouse IgG1, κ Purified isotype control (right) followed by anti-mouse IgG1 PE
  • b-S18005H_PURE_CD32B-C_Antibody_3_082119.png
    Human CD32B transfected cells were stained with CD32B (clone S18005H) Purified (filled histogram) or Mouse IgG1, κ Purified isotype control (open histogram) followed by anti-mouse IgG1 Alexa Fluor® 647.
  • c-S18005H_PURE_CD32B-C_Antibody_2_082119.png
    Human CD32A transfected cells were stained with CD32B (clone S18005H) Purified (filled histogram) or Mouse IgG1, κ Purified isotype control (open histogram) followed by anti-mouse IgG1 Alexa Fluor® 647.
Cat # Size Price Quantity Avail. Save
398302 100 µg £202
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Description

CD32B/C (FCGR2B/C) is a 40 kDa, type I transmembrane protein, member of the Ig superfamily;  the cytoplasmic tail containis an immunoreceptor tyrosine-based inhibition motif (ITIM). CD32B/C is the low affinity receptor for monomeric IgG but also bind IgG complexes. CD32B/C is expressed on B cells, subsets of monocytes,macrophages and granulocytes, platelets, mast cells, and is a negative regulator of cell activation, proliferation, endocytosis, phagocytosis, and degranulation.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant Human Fc gamma RIIB/C (CD32b/c) Protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

As the extracellular region of CD32B and CD32C are identical, this Ab recognizes both isoforms. Does not crossreact with CD32A.

RRID
AB_2814382 (BioLegend Cat. No. 398302)

Antigen Details

Structure
Type I transmembrane protein, 40 kDa, member of the Ig superfamily; the cytoplasmic tail containis an immunoreceptor tyrosine-based inhibition motif (ITIM)
Distribution

B cells, subsets of monocytes,macrophages and granulocytes, platelets, mast cells.

Function
Negatively regulates cell activation, proliferation, endocytosis, phagocytosis, and degranulation
Ligand/Receptor
Fc region of complex or aggregated immunoglobulins gamma
Cell Type
B cells, Dendritic cells, Granulocytes, Monocytes, Platelets
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, Fc Receptors
Antigen References
  1. Bruhns P, et al. 2009. Blood. 113: 3716.
  2. Bewarder N, et al. 1996. Mol Cell Biol. 16: 4735.
  3. Descours B, et al. 2017. Nature. 543: 564.
  4. Tomiyama Y, et al. 1992. Blood. 80: 2261.
  5. Indik Z, et al. 1991. J Clin Invest. 88: 1766.
  6. Ramsland PA, et al. 2011. J Immunol. 187: 3208.
  7. Hogarth PM and Pietersz GA. 2012. Nat Rev Drug Discov. 11: 311.
  8. Bournazos S, et al. 2009. J Immunol. 182: 8026.
  9. Maxwell KF, et al. 1999. Nat Struct Biol. 6: 437.
  10. Sandilands GP, et al. 1997. Immunology. 91: 204.
  11. Ghazizadeh S, et al. 1994. J Biol Chem. 269: 8878.
  12. Gillis C, et al. 2014. Front Immunol. 5: 254.
Gene ID
2213 View all products for this Gene ID 9103 View all products for this Gene ID
UniProt
View information about CD32B/C on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 08/16/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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