Purified anti-human CD31 Antibody

Pricing & Availability
Clone
WM59 (See other available formats)
Workshop
V P025
Other Names
PECAM-1, EndoCAM
Isotype
Mouse IgG1, κ
Ave. Rating
1 reviews
Product Citations
publications
WM59_FITC
Human peripheral blood lymphocytes, monocytes and granulocytes stained with purified WM59, then detected with anti-mouse IgG FITC
  • WM59_FITC
    Human peripheral blood lymphocytes, monocytes and granulocytes stained with purified WM59, then detected with anti-mouse IgG FITC
  • WM59_Purified_CD31_Antibody_ELISA_112618
  • WM59_Purified_CD31_Antibody_ICC_112018
    HUVEC cells were fixed with 1% paraformaldehyde (PFA) and blocked with 5% fetal bovine serum for 30 minutes at room temperature. Then the cells were stained with 10 µg/ml of purified anti-CD31 (clone WM59) overnight, followed by 2.5 µg/ml DyLight™ 594 anti-mouse IgG for 2 hours at 4°C (red). Nuclei were counterstained with DAPI (blue). The image was captured by 20X objective.
Cat # Size Price Quantity Avail. Save
303101 25 µg £31
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303102 100 µg £58
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Description

CD31 is a 130-140 kD type I transmembrane glycoprotein also known as platelet endothelial cell adhesion molecule-1 (PECAM-1) or Endocam. It is expressed on monocytes, platelets, granulocytes, endothelial cells and lymphocyte subsets. CD31 has been reported to bind CD38 and be involved in wound healing, angiogenesis, and cellular migration in an inflammatory situation.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, African Green, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
ELISA Capture, ICC - Validated
IHC - Reported in the literature

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume. For ELISA Capture applications, a concentration of 1.0 µg/mL is recommended. To obtain a linear standard curve, serial dilutions of CD31 recombinant protein ranging from 10 to 0.156 ng/mL is recommended for each ELISA plate. For immunocytochemistry, a concentration range of 2.5 - 10 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone WM59 has been reported to recognize the D2 extracellular portion of CD31.

Additional reported applications (for the relevant formats) include: immunofluorescence microscopy2, immunohistochemical staining of acetone-fixed frozen tissue sections8, and blocking of platelet aggregation3. Clone WM59 is not recommended for immunohistochemical staining of formalin-fixed paraffin-embedded sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 303143 & 303144).

The purified WM59 antibody is useful as a capture antibody for a sandwich ELISA assay, when used in conjunction with biotin anti-human CD31 antibody (Cat. No. 536604) antibody as the detection antibody.

Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V Oxford University Press. New York.
  2. Muczynski KA, et al. 2003. J. Am. Soc. Nephrol. 14:1336. (IF)
  3. Wu XW, et al. 1997. Arterioscl. Throm. Vas. 17:3154. (Block)
  4. Nagano M, et al. 2007. Blood 110:151. (FC) PubMed
  5. MacFadyen JR, et al. 2005. FEBS Lett. 579:2569. PubMed
  6. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  7. Sestak K, et al. 2007. Vet. Immunol. Immunopathol. 119:21.
  8. Wicki A, et al. 2012. Clin. Cancer Res. 18:454. (FC, IHC) PubMed
  9. Oeztuerk-Winder F, et al. 2012. EMBO J. 31:3431. (FC) PubMed
  10. Bushway ME, et al. 2014. Biol Reprod. 90(5): 110 (IF) PubMed
Product Citations
  1. Nagano M, et al. 2007. Blood . 110:151. PubMed
  2. Lundqvist A, et al. 2016. PLoS One. 11: 0161629. PubMed
  3. Bloodworth NC, et al. 2018. Circ Res. 123:e51. PubMed
  4. Ran LJ, et al. 2018. Mol Med Rep. 18:723. PubMed
  5. Vukusic K, et al. 2019. Stem Cells Dev. 28:1078. PubMed
  6. Yoshitomi Y, et al. 2017. J Cell Sci. 130:916. PubMed
  7. Evrard M et al. 2018. Immunity. 48(2):364-379 . PubMed
  8. Olin A et al. 2018. Cell. 174(5):1277-1292 . PubMed
  9. Robert J et al. 2017. eLife. 6 pii: e29595. PubMed
  10. Yoshihara S, et al. 2019. Front Immunol. 0.545833333. PubMed
RRID
AB_314327 (BioLegend Cat. No. 303101)
AB_314328 (BioLegend Cat. No. 303102)

Antigen Details

Structure
Ig superfamily, type I transmembrane glycoprotein, 130-140 kD
Distribution

Monocytes, platelets, granulocytes, endothelial cells, lymphocyte subset

Function
Cell adhesion, signal transduction
Ligand/Receptor
CD38
Cell Type
Endothelial cells, Granulocytes, Lymphocytes, Monocytes, Neutrophils, Platelets
Biology Area
Angiogenesis, Cell Adhesion, Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References
  1. DeLisser H, et al. 1994. Immunol. Today 15:490.
  2. Newman P, 1997. J. Clin. Invest. 99:3.
  3. Fawcett J, et al. 1995. J. Cell Biol. 128:1229.
Gene ID
5175 View all products for this Gene ID
UniProt
View information about CD31 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 07/02/2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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