Purified anti-human CD267 (TACI) Antibody

Pricing & Availability
Clone
11H3 (See other available formats)
Other Names
TACI, TNFRSF13B
Isotype
Mouse IgG2a, κ
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Product Citations
publications
11H3_PURE_CD267_Antibody_102819.png
Human peripheral blood lymphocytes were stained with CD19 APC and purified CD267 (clone 11H3) (left) or purified mouse IgG2a, κ isotype control (right), followed by anti-mouse IgG PE.
  • 11H3_PURE_CD267_Antibody_102819.png
    Human peripheral blood lymphocytes were stained with CD19 APC and purified CD267 (clone 11H3) (left) or purified mouse IgG2a, κ isotype control (right), followed by anti-mouse IgG PE.
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398602 100 µg £120
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Description

TACI, Transmembrane Activator CAML (calcium modulator and cyclophilin ligand) Interactor, is a 32 kD type III transmembrane protein. It belongs to TNF receptor superfamily, known as TNFRSF member 13B (TNFRSF13B) or CD267. TACI is expressed on B cells, predominantly CD27+ memory B cells, and myeloma cells. TACI contains 2 cysteine-rich domains (CRDs) and 1 potential N-linked glycosylation site in the extracellular domain. Recent studies, however, have shown that another shorter form (TACI_d2) of TACI exists wherein the N-terminal CRD is removed by alternative splicing. TACI_d2 contains full affinity for its ligands. Several proteins (BAFF/BLys, APRIL, Syndecan-2) have been identified as TACI ligands. The interaction of TACI with its ligands induces activation of the transcription factors NFAT, AP1, and NF-κ B. TACI also plays a crucial role in humoral immunity by negative regulation of B cell proliferation and survival.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human TACI transfected cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
Activation, WB - Reported in literature

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications include: western blot5 and  inducing of isotype switch signaling in B cells1, suppressing Ab production through TACI signaling2, inducing phosphorylation of PKA in B cells3.

Application References

(PubMed link indicates BioLegend citation)
  1. Martinez-Gallo M, et al. 2013. J Allergy Clin Immunol. 131: 468-76. (Activation)
  2. Sakurai D, et al. 2007. Eur. J Immunol. 37: 110-8. (Activation)
  3. Sakurai D, et al. 2007. Blood. 109: 2961-7. (Activation)
  4. He B, et al. 2010. Nat. Immunol. 11: 836-45. (FC)
  5. Garcia-Carmona Y, et al. 2018. Front Immunol. 9: 2125. (WB)

Antigen Details

Structure
A 32 kD type III transmembrane protein and belongs to TNF receptor superfamily, known as TNFRSF member 13B (TNFRSF13B) or CD267. TACI contains 2 cysteine-rich domains (CRDs) and 1 potential N-linked glycosylation site in the extracellular domain. Recent studies, however, have shown that another shorter form (TACI_d2) of TACI exists wherein the N-terminal CRD is removed by alternative splicing.
Distribution

Expressed on B cells, predominantly CD27+ memory B cells, and myeloma cells.

Function
Induces activation of the transcription factors NFAT, AP1, and NF-kB and plays a crucial role in humoral immunity by negative regulation of B cell proliferation and survival.
Cell Type
B cells
Biology Area
Immunology, Signal Transduction
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References
  1. Gross JA, et al. 2000. Nature 404:995.
  2. Wu Y, et al. 2000. J. Biol Chem. 275:35478.
  3. Yan M, et al. 2001. Nat. Immunol. 2:638.
  4. Hymowitz A, et al. 2005. J. Biol. Chem. 280:7218.
  5. Bischof D, et al. 2006. BLOOD. 107:3235.
Gene ID
23495 View all products for this Gene ID
UniProt
View information about CD267 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 10/25/2019

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