Purified anti-human CD262 (DR5, TRAIL-R2) Antibody

Pricing & Availability
Clone
DJR2-2 (2-6) (See other available formats)
Regulatory Status
RUO
Other Names
TRAIL-R2, KILLER, TRICK2, TNFRSF10B, Ly98, CD262
Isotype
Mouse IgG1, κ
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Product Citations
publications
DJR2-2_Purified_072007
Human peripheral blood lymphocytes stained with purified DJR2-2, followed by biotinylated anti-mouse IgG and Sav-PE
  • DJR2-2_Purified_072007
    Human peripheral blood lymphocytes stained with purified DJR2-2, followed by biotinylated anti-mouse IgG and Sav-PE
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307302 100 µg £120.00
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Description

DR5 is 55 kD member 10B of the TNF receptor superfamily (TNFRSF10B), also known as TRAIL-R2, TRICK2, KILLER, and CD262. It binds the cytotoxic ligand TRAIL and induces apoptosis. The DR5 receptor is broadly expressed on a variety of normal tissues and many tumors. DR5 expression has been reported to be upregulated in human cells by interferon-α, 2-methoxyestradiol, and paclitaxel, and downregulated by adenoviral E3 proteins.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Extracellular domain of DR5-human IgG1 Fc fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: The DJR2-2 antibody is useful for immunofluorescent staining and flow cytometric analysis of DR5/TRAIL-R2 receptor expression. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated anti-mouse IgG second step (Cat. No. 405303), followed by SAv-PE (Cat. No. 405204)).

Application References

(PubMed link indicates BioLegend citation)
  1. Uno K, et al. 2003. Blood 101:3658.
  2. Sato K, et al. 2005. J. Immunol. 174:4025.
  3. Shi J, et al. 2008. Blood 111:1309.PubMed
  4. Sonnemann J, et al. 2012. Cancer Biol Ther. 13:417. PubMed
Product Citations
  1. Shi J, et al. 2008. Blood. 111:1309. PubMed
  2. Sonnemann J, et al. 2012. Cancer Biol Ther. 13:417. PubMed
  3. Mitchell M, et al. 2017. Nat Commun. 10.1038/ncomms14179. PubMed
RRID
AB_314672 (BioLegend Cat. No. 307302)

Antigen Details

Structure
TNFR superfamily, 55 kD
Distribution
Broadly expressed on normal and tumor cells
Function
Induces apoptosis
Ligand/Receptor
TRAIL
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. MacFarlane M, et al. 1997. J. Biol. Chem. 272:25417.
2. Walczak H, et al. 1997. EMBO J. 16:5386.
3. Shigeno M, et al. 2003. Oncogene 22:1653.
4. LaVallee T, et al. 2003. Cancer Res. 63:468.
5. Nimmanapalli R, et al. 2001. Cancer Res. 61:759

Gene ID
8795 View all products for this Gene ID
UniProt
View information about CD262 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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