- RIK-2 (See other available formats)
- Regulatory Status
- Other Names
- Apo-2 ligand, Apo-2L, TNFSF10, CD253
- Mouse IgG1, κ
- Ave. Rating
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- Product Citations
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TRAIL is a 30 kD protein known as TNF-related apoptosis inducing ligand, CD253, TNFSF10, or Apo-2L. It can be expressed as a cell surface (30 kD) and soluble ligand (19-20 kD) produced by megakaryocytes and platelets, monocytes, neutrophils, NK cells and activated T cells. This antigen is also expressed on tumor cells and transformed cell lines. TRAIL has been reported to induce apoptosis in tumor and transformed cell lines by a caspase-dependent process. This antibody is useful for flow cytometric staining and blocking studies.Product Details
- Verified Reactivity
- Reported Reactivity
- African Green
- Antibody Type
- Host Species
- Human TRAIL-transfected mouse cell line
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
FC - Quality tested
Block - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for the relevant formats) include: blocking1-4 of TRAIL-induced apoptosis and T cell mediated cytotoxicity. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 308214).
(PubMed link indicates BioLegend citation)
- Kayagaki N, et al. 1999. J. Immunol. 162:2639. (Block)
- Uno K, et al. 2003. Blood 101:3658. (Block)
- Sato K, et al. 2005. J. Immunol. 174:4025. (Block)
- Denny MF, et al. 2007. Blood 110:2907. (Block)
- Kemter E, et al. 2011. Xenotransplantation. 19:40. PubMed
- Product Citations
AB_345288 (BioLegend Cat. No. 308202)
- TNF ligand superfamily member, approximately 30 kD
Widespread, highest expression in spleen, lung, prostate; NK, monocytes, neutrophils, activated T cells
- Induces apoptosis in tumorigenic and transformed cell lines, involved in T cell mediated cytotoxicity, TRAIL ligand of cognate receptors induces NF-κB signaling in a variety of cell types
- DR4 and DR5 cognate receptors, also binds to the decoy receptors DcR1 and DcR2
- Cell Type
- Monocytes, Neutrophils, NK cells, T cells
- Biology Area
- Molecular Family
- CD Molecules
- Antigen References
1. Pitti RM, et al. 1996. J. Biol. Chem. 271:12687.
2. Mariani SM, et al. 1998. Eur. J. Immunol. 28:973.
3. Dorothee G, et al. 2002. J. Immunol. 169:809.
4. Crist SA, et al. 2004. Exp. Hematology 32:1073.
- Gene ID
- 8743 View all products for this Gene ID
- View information about CD253 on UniProt.org
Related Pages & Pathways
Other FormatsView All CD253 Reagents Request Custom Conjugation
|Purified anti-human CD253 (TRAIL)||RIK-2||FC,Block|
|PE anti-human CD253 (TRAIL)||RIK-2||FC|
|APC anti-human CD253 (TRAIL)||RIK-2||FC|
|TotalSeq™-A0803 anti-human CD253 (Trail)||RIK-2||PG|
|Ultra-LEAF™ Purified anti-human CD253 (Trail)||RIK-2||FC,Block|
|PE/Cyanine7 anti-human CD253 (Trail)||RIK-2||FC|
|TotalSeq™-C0803 anti-human CD253 (Trail)||RIK-2||PG|
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