- ML5 (See other available formats)
- Regulatory Status
- V CD24.5
- Other Names
- Ly-52, Heat Stable Antigen (HSA), Nectadrin, BA-1
- Mouse IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
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CD24 is a 35-45 kD glycosylphosphatidylinositol (GPI)-linked protein also known as heat stable antigen (HSA), BA-1, Ly-52, and nectadrin. It is expressed on the surface of B cells (but not plasma cells), granulocytes, follicular dendritic cells, and epithelial cells. CD24 may play a role in the regulation of B-cell proliferation and maturation. CD24 crosslinking induces a Ca2+ flux in mature B cells. CD24 has been shown to interact with CD62P (P-selectin).Product Details
- Human, Cross-Reactivity: Chimpanzee
- Antibody Type
- Host Species
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.
- The antibody was purified by affinity chromatography.
- 1.0 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
FC - Quality tested
CyTOF® - Verified
- Recommended Usage
This product is suitable for use with the Maxpar® Metal Labeling Kits. The product is formulated to simplify the antibody preparation when performing the labeling protocol. As a result, it is possible to proceed directly to the step to Partially Reduce the Antibody by adding 100 µl of Maxpar® Ready antibody to 100 µl of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar® User Guide when conjugating Maxpar® Ready antibodies.
- Application Notes
Additional reported applications (for the relevant formats) include: immunofluorescence microscopy3.
- Additional Product Notes
Maxpar® is a registered trademark of Fluidigm Inc.
(PubMed link indicates BioLegend citation)
- Schlossman S, et al. Eds. 1995. Leukocyte Typing V:White Cell Differentiation Antigens. Oxford University Press. New York.
- McMichael A, et al. 1987. Leucocyte Typing III. Oxford University Press. New York.
- Yang GP, et al. 1999. Nucleic Acids Research 27:1517. (IF)
- Kristiansen G, et al. 2003. Clin. Cancer Res. 9:4906. (FC)
- Product Citations
AB_2563733 (BioLegend Cat. No. 311127)
- GPI-linked glycoprotein, 35-45 kD
B cells, granulocytes, epithelial cells
- B cell proliferation and differentiation
- CD62P (P-Selectin)
- Cell Type
- B cells, Epithelial cells, Granulocytes
- Biology Area
- Molecular Family
- CD Molecules
- Antigen References
1. Schlossman S, et al. Eds. 1995. Leukocyte Typing V. Oxford University Press. New York.
- Gene ID
- 100133941 View all products for this Gene ID
- View information about CD24 on UniProt.org
- Can I obtain CyTOF data related to your Maxpar® Ready antibody clones?
We do not test our antibodies by mass cytometry or on a CyTOF machine in-house. The data displayed on our website is provided by Fluidigm®. Please contact Fluidigm® directly for additional data and further details.
- Can I use Maxpar® Ready format clones for flow cytometry staining?
We have not tested the Maxpar® Ready antibodies formulated in solution containing EDTA for flow cytometry staining. While it is likely that this will work in majority of the situations, it is best to use the non-EDTA formulated version of the same clone for flow cytometry testing. The presence of EDTA in some situations might negatively affect staining.
- I am having difficulty observing a signal after conjugating a metal tag to your Maxpar® antibody. Please help troubleshoot.
We only supply the antibody and not test that in house. Please contact Fluidigm® directly for troubleshooting advice: http://techsupport.fluidigm.com/
- Is there a difference between buffer formulations related to Maxpar® Ready and purified format antibodies?
The Maxpar® Ready format antibody clones are formulated in Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA. The regular purified format clones are formulated in solution that does not contain any EDTA. Both formulations are however without any extra carrier proteins.
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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