Purified anti-human CD127 IL-7Ralpha Maxpar Ready Antibody anti-CD127

Pricing & Availability

Clone: A019D5 (See other available formats)
Regulatory Status: RUO
Other Names: IL-7 receptor α chain, IL-7Rα
Isotype: Mouse IgG1, κ
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Product Citations: publications

Human PBMCs stained with 154Sm-anti-CD3 (UCHT1) and 143Nd-anti-CD127 (A019D5). CD45+ CD33- cells are displayed in the analysis. Data provided by DVS Sciences.

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351337

100 µg

£101

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Description

CD127 is a 60-90 kD type I transmembrane glycoprotein also known as IL-7 receptor α chain or IL-7Rα. It forms a heterodimer with the common γ chain (γc or CD132) which is shared with the receptors for IL-2, IL-4, IL-9, IL-13, IL-15, and IL-21. CD127 is expressed on immature B cells through early pre-B stage cells, thymocytes (except CD4/CD8 double positive thymocytes), peripheral T cells, and bone marrow stromal cells. CD127 has been reported to be a useful marker for identifying memory and effector T cells. Studies have shown that CD127 expression is down-modulated on Treg cells. It can be used as a marker for differentiation of Treg and conventional T cells. The ligation of IL-7 with its receptor is important for stimulation of mature and immature T cells as well as immature B cell proliferation and development.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity

Human

Reported Reactivity

African Green, Baboon, Cynomolgus, Rhesus

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Recombinant human CD127

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.

Preparation

The antibody was purified by affinity chromatography.

Concentration

1.0 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C.

Application

FC - Quality tested
CyTOF®, PG - Verified

Recommended Usage

This product is suitable for use with the Maxpar® Metal Labeling Kits. For metal labeling using Maxpar® Ready antibodies, proceed directly to the step to Partially Reduce the Antibody by adding 100 µl of Maxpar® Ready antibody to 100 µl of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar® User Guide when conjugating Maxpar® Ready antibodies.

Application Notes

Additional reported (for the relevant formats) application: proteogenomics¹.

Additional Product Notes

Maxpar® is a registered trademark of Standard BioTools Inc.

Application References (PubMed link indicates BioLegend citation)

Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)

Product Citations

Roussel M, et al. 2021. Cell Reports Medicine. 2(6):100291. PubMed

RRID

AB_2563715 (BioLegend Cat. No. 351337)

Antigen Details

Structure: Type I transmembrane glycoprotein, associates with CD132, 60-90 kD
Distribution: Immature B cells through early pre-B stage, thymocytes (except CD4/CD8 double positive thymocytes), peripheral T cells, bone marrow stromal cells
Function: T cell and immature B cell proliferation and development
Ligand/Receptor: IL-7
Cell Type: B cells, T cells, Thymocytes, Tregs
Biology Area: Immunology
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors
Antigen References: 1. Sudo T, et al. 1993. P. Natl. Acad. Sci. USA 90:9125.
2. He YW and Malek TR. 1998. Crit. Rev. Immunol. 18:503.
3. Huster KM, et al. 2004. P. Natl. Acad. Sci. USA 101:5610.
4. Pillai M, et al. 2004. Leukemia Lymphoma 45:2403.
5. Morrissey PJ, et al. 1989. J. Exp. Med. 169:707.
6. Liu W, et al. 2006. J. Exp. Med. 203:1701.
Gene ID: 3575 View all products for this Gene ID
UniProt: View information about CD127 on UniProt.org

Documentation

Reviews

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Related Pages & Pathways

Pathways

Related FAQs

Can I obtain CyTOF data related to your Maxpar® Ready antibody clones?

We do not test our antibodies by mass cytometry or on a CyTOF machine in-house. The data displayed on our website is provided by Fluidigm^®. Please contact Fluidigm^® directly for additional data and further details.

http://techsupport.fluidigm.com/

Can I use Maxpar® Ready format clones for flow cytometry staining?

We have not tested the Maxpar^® Ready antibodies formulated in solution containing EDTA for flow cytometry staining. While it is likely that this will work in majority of the situations, it is best to use the non-EDTA formulated version of the same clone for flow cytometry testing. The presence of EDTA in some situations might negatively affect staining.

I am having difficulty observing a signal after conjugating a metal tag to your Maxpar® antibody. Please help troubleshoot.

We only supply the antibody and not test that in house. Please contact Fluidigm^® directly for troubleshooting advice: http://techsupport.fluidigm.com/

Is there a difference between buffer formulations related to Maxpar® Ready and purified format antibodies?

The Maxpar^® Ready format antibody clones are formulated in Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA. The regular purified format clones are formulated in solution that does not contain any EDTA. Both formulations are however without any extra carrier proteins.

Other Formats

View All CD127 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-human CD127 (IL-7Rα)	A019D5	FC
PE anti-human CD127 (IL-7Rα)	A019D5	FC
Pacific Blue™ anti-human CD127 (IL-7Rα)	A019D5	FC
Brilliant Violet 421™ anti-human CD127 (IL-7Rα)	A019D5	FC
FITC anti-human CD127 (IL-7Rα)	A019D5	FC
Alexa Fluor® 488 anti-human CD127 (IL-7Rα)	A019D5	FC
APC anti-human CD127 (IL-7Rα)	A019D5	FC
Alexa Fluor® 647 anti-human CD127 (IL-7Rα)	A019D5	FC
PE/Cyanine7 anti-human CD127 (IL-7Rα)	A019D5	FC
PerCP/Cyanine5.5 anti-human CD127 (IL-7Rα)	A019D5	FC
Brilliant Violet 570™ anti-human CD127 (IL-7Rα)	A019D5	FC
PE/Cyanine5 anti-human CD127 (IL-7Rα)	A019D5	FC
Brilliant Violet 650™ anti-human CD127 (IL-7Rα)	A019D5	FC
Brilliant Violet 711™ anti-human CD127 (IL-7Rα)	A019D5	FC
Brilliant Violet 785™ anti-human CD127 (IL-7Rα)	A019D5	FC
Brilliant Violet 510™ anti-human CD127 (IL-7Rα)	A019D5	FC
Brilliant Violet 605™ anti-human CD127 (IL-7Rα)	A019D5	FC
PE/Dazzle™ 594 anti-human CD127 (IL-7Rα)	A019D5	FC
Purified anti-human CD127 (IL-7Rα) (Maxpar® Ready)	A019D5	FC,CyTOF®
Alexa Fluor® 700 anti-human CD127 (IL-7Rα)	A019D5	FC
Biotin anti-human CD127 (IL-7Rα)	A019D5	FC
APC/Cyanine7 anti-human CD127 (IL-7Rα)	A019D5	FC
APC/Fire™ 750 anti-human CD127 (IL-7Rα)	A019D5	FC
TotalSeq™-A0390 anti-human CD127 (IL-7Rα)	A019D5	PG
TotalSeq™-B0390 anti-human CD127 (IL-7Rα)	A019D5	PG
TotalSeq™-C0390 anti-human CD127 (IL-7Rα)	A019D5	PG
KIRAVIA Blue 520™ anti-human CD127 (IL-7Rα)	A019D5	FC
Spark NIR™ 685 anti-human CD127 (IL-7Rα)	A019D5	FC
PE/Fire™ 640 anti-human CD127 (IL-7Rα)	A019D5	FC
PE/Fire™ 700 anti-human CD127 (IL-7Rα) Antibody	A019D5	FC
Spark YG™ 581 anti-human CD127 (IL-7Rα)	A019D5	FC
Brilliant Violet 750™ anti-human CD127 (IL-7Rα)	A019D5	FC
TotalSeq™-D0390 anti-human CD127 (IL-7Rα)	A019D5	PG
APC/Fire™ 810 anti-human CD127 (IL-7Rα) Antibody	A019D5	FC
APC/Fire™ 750 anti-human CD127	A019D5	FC
PE anti-human CD127	A019D5	FC
PerCP/Cyanine5.5 anti-human CD127	A019D5	FC
PE/Cyanine7 anti-human CD127	A019D5	FC
Spark Red™ 718 anti-human CD127 (IL-7Rα)	A019D5	FC
GMP PE/Cyanine7 anti-human CD127 (IL-7Rα)	A019D5	FC

Certificate of Analysis

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Go To Top Version: 2 Revision Date: 07/22/2022

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.