- 3.9 (See other available formats)
- Regulatory Status
- III NL707
- Other Names
- Integrin αX subunit, CR4, p150, ITGAX
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
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CD11c is a 145-150 kD type I transmembrane glycoprotein also known as integrin αX and CR4. CD11c non-covalently associates with integrin β2 (CD18) and is expressed on monocytes/macrophages, dendritic cells, granulocytes, NK cells, and subsets of T and B cells. CD11c has been reported to play a role in adhesion and CTL killing through its interactions with fibrinogen, CD54, and iC3b.Product Details
- Verified Reactivity
- Human, Cynomolgus, Rhesus
- Reported Reactivity
- African Green, Baboon, Chimpanzee, Squirrel Monkey
- Antibody Type
- Host Species
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.
- The antibody was purified by affinity chromatography.
- 1.0 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
FC - Quality tested
CyTOF® - Verified
- Recommended Usage
This product is suitable for use with the Maxpar® Metal Labeling Kits. For metal labeling using Maxpar® Ready antibodies, proceed directly to the step to Partially Reduce the Antibody by adding 100 µl of Maxpar® Ready antibody to 100 µl of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar® User Guide when conjugating Maxpar® Ready antibodies.
- Application Notes
Clone 3.9 preferentially binds the activated form of CD11c, is specific for the I domain of CD11c, and is able to partially block the binding of CD11c and ICAM-4. 3.9 binding is divalent cation dependent12. While analyzing blood, it is best to use heparin as the anti-coagulant and not EDTA. Since the ability of clone 3.9 to bind to its target is divalent cation dependent, the usage of EDTA as an anti-coagulant may be detrimental to staining due to its chelating properties.
Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections4, and functional assays5,6. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 301616). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 301632) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).
- Additional Product Notes
Maxpar® is a registered trademark of Standard BioTools Inc.
(PubMed link indicates BioLegend citation)
- Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
- Knapp W, et al. 1989. Leucocyte Typing IV Oxford University Press. New York.
- McMichael A, et al. Eds. 1987. Leucocyte Typing III Oxford University Press. New York.
- Vainer B, et al. 2000. Am. J. Surg. Pathol. 24:1115. (IHC)
- Ottonello L, et al. 1999. Blood 93:3505.
- Metelitsa LS, et al. 2002. Blood 99:4166.
- Sadhu C, et al. 2007. J. Leukoc. Biol. doi:10.1189/jlb.1106680. PubMed
- Ihanus E, et al. 2007. Blood 109:802-810.
- Gurer C, et al. 2008. Blood 112:1231. PubMed
- Asai A, et al. 2009. J. Lipid Res. 50:95. PubMed
- Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
- Sadhu C, et al. 2008. J. Immunoass. Immunoch. 29:42. (FC)
- Product Citations
AB_2562812 (BioLegend Cat. No. 301639)
- Integrin, type I transmembrane glycoprotein, associates with integrin β2 (CD18), 145-150 kD
Myeloid, dendritic cells, NK cells, B cells and T cell subsets
- Adhesion, CTL killing
- CD54, fibrinogen, iC3b, ICAM-1, ICAM-4
- Cell Type
- B cells, Dendritic cells, Neutrophils, NK cells, T cells, Tregs
- Biology Area
- Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
1. Petty H. 1996. Immunol. Today 17:209.
2. Springer T. 1994. Cell 76:301.
3. Ihanus E, et al. 2007. Blood 109:802-810.
- Gene ID
- 3687 View all products for this Gene ID
- View information about CD11c on UniProt.org
- Can I obtain CyTOF data related to your Maxpar® Ready antibody clones?
We do not test our antibodies by mass cytometry or on a CyTOF machine in-house. The data displayed on our website is provided by Fluidigm®. Please contact Fluidigm® directly for additional data and further details.
- Can I use Maxpar® Ready format clones for flow cytometry staining?
We have not tested the Maxpar® Ready antibodies formulated in solution containing EDTA for flow cytometry staining. While it is likely that this will work in majority of the situations, it is best to use the non-EDTA formulated version of the same clone for flow cytometry testing. The presence of EDTA in some situations might negatively affect staining.
- I am having difficulty observing a signal after conjugating a metal tag to your Maxpar® antibody. Please help troubleshoot.
We only supply the antibody and not test that in house. Please contact Fluidigm® directly for troubleshooting advice: http://techsupport.fluidigm.com/
- Is there a difference between buffer formulations related to Maxpar® Ready and purified format antibodies?
The Maxpar® Ready format antibody clones are formulated in Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA. The regular purified format clones are formulated in solution that does not contain any EDTA. Both formulations are however without any extra carrier proteins.
|FITC anti-human CD11c||3.9||FC|
|PE anti-human CD11c||3.9||FC|
|Purified anti-human CD11c||3.9||FC, CyTOF®, IHC|
|PE/Cyanine7 anti-human CD11c||3.9||FC|
|PE/Cyanine5 anti-human CD11c||3.9||FC|
|Biotin anti-human CD11c||3.9||FC|
|APC anti-human CD11c||3.9||FC|
|Alexa Fluor® 488 anti-human CD11c||3.9||FC|
|Alexa Fluor® 647 anti-human CD11c||3.9||FC|
|Pacific Blue™ anti-human CD11c||3.9||FC|
|PerCP/Cyanine5.5 anti-human CD11c||3.9||FC|
|Brilliant Violet 421™ anti-human CD11c||3.9||FC|
|Brilliant Violet 711™ anti-human CD11c||3.9||FC|
|Ultra-LEAF™ Purified anti-human CD11c||3.9||FC, CyTOF®, Block, IHC|
|Brilliant Violet 510™ anti-human CD11c||3.9||FC, ICC|
|Brilliant Violet 605™ anti-human CD11c||3.9||FC|
|Brilliant Violet 650™ anti-human CD11c||3.9||FC|
|Purified anti-human CD11c (Maxpar® Ready)||3.9||FC, CyTOF®|
|PE/Dazzle™ 594 anti-human CD11c||3.9||FC|
|Brilliant Violet 785™ anti-human CD11c||3.9||FC|
|Alexa Fluor® 700 anti-human CD11c||3.9||FC|
|APC/Fire™ 750 anti-human CD11c||3.9||FC|
|Spark Red™ 718 anti-human CD11c||3.9||FC|
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
FITC anti-human CD11c
PE anti-human CD11c
Purified anti-human CD11c
PE/Cyanine7 anti-human CD11c
PE/Cyanine5 anti-human CD11c
Biotin anti-human CD11c
APC anti-human CD11c
Alexa Fluor® 488 anti-human CD11c
Alexa Fluor® 647 anti-human CD11c
Pacific Blue™ anti-human CD11c
PerCP/Cyanine5.5 anti-human CD11c
Brilliant Violet 421™ anti-human CD11c
Brilliant Violet 711™ anti-human CD11c
Ultra-LEAF™ Purified anti-human CD11c
Brilliant Violet 510™ anti-human CD11c
Brilliant Violet 605™ anti-human CD11c
Brilliant Violet 650™ anti-human CD11c
Purified anti-human CD11c (Maxpar® Ready)
PE/Dazzle™ 594 anti-human CD11c
Brilliant Violet 785™ anti-human CD11c
Alexa Fluor® 700 anti-human CD11c
APC/Fire™ 750 anti-human CD11c
Spark Red™ 718 anti-human CD11c