Purified anti-human BDNF Antibody

Pricing & Availability
Clone
A15062G (See other available formats)
Other Names
Brain-derived neurotrophic factor, ANON2, BLUN2
Isotype
Mouse IgG1
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536702 500 µg £333
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Description

Brain-derived neurotrophic factor (BDNF) is the second member of the NGF family of neurotrophic factors (neurotrophins), which includes nerve growth factor (NGF), neurotrophin-3 (NT-3), and neurotrophin-4/5 (NT-4/5). BDNF shares approximately 55% amino acid identity with other family members. The amino acid sequence of mature BDNF is identical in all mammalian species examined to date. Mature BDNF promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems during development. BDNF participates in axonal growth, pathfinding, and in the modulation of dendritic growth and morphology. BDNF is also a major regulator of synaptic transmission and plasticity at adult synapses in many regions of the central nervous system (CNS). High levels of expression of BDNF have been detected in the hippocampus, cerebellum, fetal eye, and placenta. BDNF binds with high affinity and specifically activates the TrkB tyrosine kinase receptor. BDNF is also associated with multiple diseases such as Schizophrenia, Rett syndrome, obsessive compulsive disorder, Huntington’s disease, eating disorders, depression, dementia and Alzheimer’s disease. For example, BDNF levels are decreased in the serum of Alzheimer’s disease patients.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ELISA Capture - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA Capture applications, a concentration range of 1 - 4 µg/mL is recommended. To obtain a linear standard curve, serial dilutions of BDNF recombinant protein ranging from 156.3 to 10,000 pg/mL is recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The purified A15062G antibody is useful as the capture antibody in a sandwich ELISA assay, when used in conjunction with the biotinylated A15062F antibody (Cat. No. 536804) as the detection antibody and Recombinant Human BDNF (ELISA Std.) (Cat. No. 759709).

Although not tested, additional species reactivity for rat, donkey, equine, porcine, and monkey are predicted based on 100% amino acid sequence conservation for this target across listed species.

RRID
AB_2783342 (BioLegend Cat. No. 536702)

Antigen Details

Structure
Noncovalently linked homodimer.
Distribution

Ubiquitous with high levels in peripheral tissues including prostate, placenta, pancreas, heart, kidney, pituitary gland, lung, and testis.

Function
Supports the survival of sensory and sympathetic neurons, and also dopaminergic midbrain neurons.
Interaction
Sensory neurons, sympathetic neurons, midbrain dopaminergic neurons
Ligand Receptor
TrkB
Antigen References
  1. Baloh RH, et al. 1998. Neuron. 21:1291.
  2. Masure S, et al. 1999. Eur. J. Biochem. 266:892-902.
  3. Gardell LR, et al. 2003. Nat. Med. 9(11):1383-9.
  4. Honma Y, et al. 2002. Neuron. 35:267-82.
  5. Banerjee A, et al. 2012. PLoS One 7(11):e50098.
  6. Wong LE, et al. 2015. Proc. Natl. Acad. Sci. USA 112:6170-5.
  7. Zhang M, et al. 2016. Oncotarget. 7:3267-82.
Gene ID
627 View all products for this Gene ID
UniProt
View information about BDNF on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 12/10/2018

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