Purified anti-HSD17B10 Antibody (Previously Covance catalog# SIG-39770)

Pricing & Availability
Clone
5F3 (See other available formats)
Other Names
17b-HSD10, ABAD, CAMR, DUPXp11.22, HADH2, HCD2, MHBD, MRPP2, MRX17, MRX31, MRXS10, SCHAD, SDR5C1, HSD17B10, HSD-10, ABAD, 3-hydroxyacyl-CoA dehydrogenase type-2, AB-binding alcohol dehydrogenase, mitochondrial ribonuclease P protein 2, 3-hydroxy-2-methylbutyryl-CoA dehydrogenase, short chain type dehydrogenase/reductase XH98G2, amyloid-beta peptide binding alcohol dehydrogen
Previously
Signet Catalog# 9770-02
Signet Catalog# 9770-05
Signet Catalog# 9770-10
Covance Catalog# SIG-39770
Isotype
Mouse IgG1, λ
Ave. Rating
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Product Citations
publications
5F3_Purified_HSD17B10_Antibody_IHC_021315
Mitochondrial localization of HSD17B10 in human neuroblastoma cells SK-N-SH visualized with MAb 5F3 and laser-scanning confocal microscope.
A. MAb to mitochondria visualized with Cy-5-conjugated secondary antibodies.
B. MAb 5F3 to HSD17B10-FITC-conjugated.
C. Merged image.
  • 5F3_Purified_HSD17B10_Antibody_IHC_021315
    Mitochondrial localization of HSD17B10 in human neuroblastoma cells SK-N-SH visualized with MAb 5F3 and laser-scanning confocal microscope.
    A. MAb to mitochondria visualized with Cy-5-conjugated secondary antibodies.
    B. MAb 5F3 to HSD17B10-FITC-conjugated.
    C. Merged image.
  • 5F3_Purified_HSD17B10_Antibody_IHC_1_021315
    IHC staining of purified anti-HSD17B10 antibody (clone 5F3) on formalin-fixed paraffin-embedded human colon tissue. The tissue was incubated with 10 µg/ml of the primary antibody for 60 minutes at room temperature. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
Cat # Size Price Quantity Avail. Save
834405 25 µl £60
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834404 100 µl £158
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834402 500 µl £333
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Description

The clone 5F3 recognizes the endogenous HSD17B10 protein in extracts from cells with virtually no cross-reactivity to other human proteins in immunoblots and ELISA assays. HSD17B10 is an intracellular 27kD polypeptide which appears to be up regulated in the brains of Alzheimer’s disease patients and has been associated with amyloid beta neurotoxicity. Cross-reactivity to mouse or other species has not been tested.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This antibody was generated against recombinant, full-length human HSD17B10 protein.
Formulation
Phosphate-buffered solution; no preservatives or carrier proteins.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

IHC-P - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone 5F3 recognizes the recombinant human HSD17B10 protein (27kD) and is an excellent mitochondrial marker.

Please note that the name of this product has changed from Purified anti-ERAB Antibody to Purified anti-HSD17B10 Antibody.

Application References

(PubMed link indicates BioLegend citation)
  1. He XY, et al. 2000. Biochim BioPhys Acta. 1484 (2-3):267-77.
  2. Yan SD, et al. 1999. J Biol Chem. 274(4):2145-56.
  3. Oppermann UC, et al. 1999. FEBS Lett. 451(3):238-42.
  4. Sambamurti K & Lahiri DK. 1998. Biochem Biophys Res Commun. 249(2):546-9.
  5. Yan SD, et al. 1997. Nature. 389(6652):689-95.
  6. Furuta S, et al. 1997. Biochim BioPhys Acta. 1350(3):317-24.
RRID
AB_2783409 (BioLegend Cat. No. 834405)
AB_2783408 (BioLegend Cat. No. 834404)
AB_2564990 (BioLegend Cat. No. 834402)

Antigen Details

Biology Area
Cell Biology, Mitochondrial Function, Neuroscience, Neuroscience Cell Markers, Signal Transduction
Molecular Family
Mitochondrial Markers
Gene ID
3028 View all products for this Gene ID
UniProt
View information about HSD17B10 on UniProt.org

Related FAQs

Can I an isotype control with a lambda light chain be substituted with an isotype control with a kappa light chain for flow cytometry?

Yes, you can since kappa and lambda represent light chains which don't contribute to the background staining.

Go To Top Version: 4    Revision Date: 09/06/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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