Purified anti-HPR Antibody

Pricing & Availability
Clone
BL28632 (See other available formats)
Other Names
Haptoglobin-Related Protein, Haptoglobin-Related Locus, A-259H10.2
Isotype
Mouse IgG2b, κ
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Product Citations
publications
BL28632_Purified_HPR_Antibody_1_062519
Western blot of purified anti-HPR antibody (clone BL28632). Lane 1: Molecular weight marker; Lane 2: 20 µg of human kidney lysate. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • BL28632_Purified_HPR_Antibody_1_062519
    Western blot of purified anti-HPR antibody (clone BL28632). Lane 1: Molecular weight marker; Lane 2: 20 µg of human kidney lysate. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • BL28632_Purified_HPR_Antibody_2_062519
    IHC staining of purified anti-HPR antibody (clone BL28632) on formalin-fixed paraffin-embedded human kidney tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928502), the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
Cat # Size Price Quantity Avail. Save
863201 25 µg £60
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Description

Haptoglobin-Related Protein (HPR) is a primate-specific plasma protein that binds to hemoglobin as efficiently as haptoglobin. HPR is also involved in human innate immune protection through its association with high-density lipoprotein (HDL). It may contribute to the clearance of cell-free hemoglobin to allow hepatic recycling of heme iron. HPR is associated with diseases including anhaptoglobinemia and sleeping sickness, and may serve as an important predictor of recurrence of breast cancer.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant HPR expressed in E.coli.
Formulation
0.01 M PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
Store at 4°C for up to 3 months. For longer term storage aliquot into small volumes and store at -20°C.
Application

WB - Quality tested
IHC-P - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.2 - 1.0 µg per ml. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Antigen Details

Structure
HPR is a 348 amino acid protein with an apparent molecular mass of ~ 39 kD.
Distribution

Tissue Distribution: Liver and kidney
Cellular Distribution: Cytosol

Function
HPR is involved in the clearance of cell-free hemoglobin to allow hepatic recycling of heme iron.
Interaction
Hemoglobin
Cell Type
Mesenchymal cells
Biology Area
Cancer Biomarkers, Immuno-Oncology, Immunology, Innate Immunity
Molecular Family
Carrier Proteins, Innate Immune Signaling
Antigen References
  1. Nielsen MJ, et al. 2009. Blood. 114(4):764.
  2. Martin-Lorenzo M, et al. 2019. Hypertension. 73(4):794.
Gene ID
3250 View all products for this Gene ID
UniProt
View information about HPR on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 06/25/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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