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Purified anti-HPR Antibody

Pricing & Availability
Clone
BL28632 (See other available formats)
Regulatory Status
RUO
Other Names
Haptoglobin-Related Protein, Haptoglobin-Related Locus, A-259H10.2
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
BL28632_Purified_HPR_Antibody_1_062519
Western blot of purified anti-HPR antibody (clone BL28632). Lane 1: Molecular weight marker; Lane 2: 20 µg of human kidney lysate. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • BL28632_Purified_HPR_Antibody_1_062519
    Western blot of purified anti-HPR antibody (clone BL28632). Lane 1: Molecular weight marker; Lane 2: 20 µg of human kidney lysate. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • BL28632_Purified_HPR_Antibody_2_062519
    IHC staining of purified anti-HPR antibody (clone BL28632) on formalin-fixed paraffin-embedded human kidney tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928502), the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
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863201 25 µg £73
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Description

Haptoglobin-Related Protein (HPR) is a primate-specific plasma protein that binds to hemoglobin as efficiently as haptoglobin. HPR is also involved in human innate immune protection through its association with high-density lipoprotein (HDL). It may contribute to the clearance of cell-free hemoglobin to allow hepatic recycling of heme iron. HPR is associated with diseases including anhaptoglobinemia and sleeping sickness, and may serve as an important predictor of recurrence of breast cancer.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant HPR expressed in E.coli.
Formulation
0.01 M PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
Store at 4°C for up to 3 months. For longer term storage aliquot into small volumes and store at -20°C.
Application

WB - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.2 - 1.0 µg per ml. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2810757 (BioLegend Cat. No. 863201)

Antigen Details

Structure
HPR is a 348 amino acid protein with an apparent molecular mass of ~ 39 kD.
Distribution

Tissue Distribution: Liver and kidney
Cellular Distribution: Cytosol

Function
HPR is involved in the clearance of cell-free hemoglobin to allow hepatic recycling of heme iron.
Interaction
Hemoglobin
Cell Type
Mesenchymal cells
Biology Area
Cancer Biomarkers, Immuno-Oncology, Immunology, Innate Immunity
Molecular Family
Carrier Proteins, Innate Immune Signaling
Antigen References
  1. Nielsen MJ, et al. 2009. Blood. 114(4):764.
  2. Martin-Lorenzo M, et al. 2019. Hypertension. 73(4):794.
Gene ID
3250 View all products for this Gene ID
UniProt
View information about HPR on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 06/25/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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