Purified anti-Histone H3 (C-terminus) Antibody

Pricing & Availability
Clone
1B1-B2 (See other available formats)
Other Names
Histone H3.1, histone H3/a, histone 1, H3a, H3 histone family, member A, H3/A, H3FA
Isotype
Mouse IgG3
Ave. Rating
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Product Citations
publications
1_1B1-B2_Purified_Histone_H3_CO2_Terminus_Antibody_IHC_030415.jpg
IHC staining of purified anti-Histone H3 (C-Terminus) antibody (clone 1B1-B2) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/mL of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective.
  • 1_1B1-B2_Purified_Histone_H3_CO2_Terminus_Antibody_IHC_030415.jpg
    IHC staining of purified anti-Histone H3 (C-Terminus) antibody (clone 1B1-B2) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/mL of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective.
  • 2_1B1-B2_PURE_a-HistoneH3_IHC_Antibody_2_043018.png
    IHC staining of purified anti-Histone H3 (C-Terminus) antibody (clone 1B1-B2) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/mL of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • 3_1B1-B2_PURE_a-HistoneH3_IHC_Antibody_3_043018.png
    IHC staining of purified anti-Histone H3 (C-Terminus) antibody (clone 1B1-B2) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/mL of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • 4_1B1-B2_PURE_Histone_H3_Antibody_4_WB_102519_updated
    Western blot of purified anti-Histone H3 (C-terminus) antibody (clone 1B1-B2). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20µg of rat brain lysate; Lane 5: 10µg of Drosophila head lysate. The blot was incubated with 0.1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP Goat anti-mouse IgG Antibody (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • 5_1B1-B2_PURE_Histone_H3_Antibody_2_WB_051519
    Western blot of purified anti-Histone H3 (C-terminus) antibody (clone 1B1-B2). Lane 1: Molecular weight marker; Lane 2: 20 µg of Drosophila head lysate; Lane 3: 20 µg of Drosophila S2 (embryonic) cell lysate. The blot was incubated with 0.1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP Goat anti-mouse IgG Antibody (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
Cat # Size Price Quantity Avail. Save
819411 25 µg £50
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819412 100 µg £155
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Description

Histone proteins are classified into core histones (H2A, H2B, H3, H4) and linker histones (H1, H5). Core histones form an octamer, which contains two H2A-H2B dimers and one H3-H4 tetramer. Core histones are predominantly globular except for the unstructured N-terminal tails. Posttranslational modifications, such as acetylation, methylation, phosphorylation, ubiquitination, SUMOylation and ADP-ribosylation occur in histone tails.

Histone modifications induce changes of chromatin structure and thereby affect the accessibility of transcription factors, nuclear proteins and enzymes to genomic DNA, resulting in gene activation or repression. It is known that histone modifications play critical roles in DNA repair, DNA replication, transcription regulation, alternative splicing and chromosome condensation and some diseases including autoimmune diseases and cancers.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse, Rat, Drosophila
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against a peptide sequence derived from the C-terminal domain of human Histone H3 protein.
Formulation
50 mM sodium citrate, 150mM sodium chloride, pH 5.8 with stabilizer
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
WB - Validated

Recommended Usage

Each lot of this antibody is quality control tested by immunohistochemistry. For immunohistochemistry, a concentration of 1.0 µg/mL is suggested. For Western blotting, the suggested use of this reagent is 0.01 - 0.5 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Reactivity to Drosophila was only validated with the purified format.

Additional Product Notes

For Western blotting, the suggested use of this reagent is 0.01 - 0.2 µg/mL in human, mouse, and rat and 0.1 - 0.5 µg/mL in Drosophila.

Antigen Details

Structure
Histone proteins H3 and H4 bind to form a H3-H4 dimer, two of these H3-H4 dimers combine to form a tetramer. This tetramer further combines with two H2a-H2b dimers to form the compact Histone octamer core.
Distribution

Nucleus

Function
Histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability.
Interaction
Rsk-2, MSK1, GCN5 family of HATs, HDACs, PARP, SET7/9, and CARM1
Biology Area
Cell Biology, Chromatin Remodeling/Epigenetics, Neuroscience, Transcription Factors
Antigen References

1. Chen HM, et al. 2015. Epigenetics and Dermatology 409.

Gene ID
8350 View all products for this Gene ID
UniProt
View information about Histone H3 CO2-terminus on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 10/24/2019

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