Purified anti-HCN4 Antibody

Pricing & Availability
Clone
N114/10 (See other available formats)
Other Names
Potassium sodium hyperpolarization-activated cyclic nucleotide-gated channel 4, brain cyclic nucleotide-gated channel 3 and BCNG-3
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
N114-10_purified_HCN4_Antibody_1_112018
Western blot of purified anti-HCN4 antibody (clone N114/10). Lane 1: Molecular weight marker; Lane 2: 20 µg of mouse brain membrane lysate; Lane 3: 20 µg of rat brain membrane lysate. The blot was incubated with 2.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system. The doublet of bands represent phosphorylated and nonphosphorylated HCN4 proteins.
  • N114-10_purified_HCN4_Antibody_1_112018
    Western blot of purified anti-HCN4 antibody (clone N114/10). Lane 1: Molecular weight marker; Lane 2: 20 µg of mouse brain membrane lysate; Lane 3: 20 µg of rat brain membrane lysate. The blot was incubated with 2.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system. The doublet of bands represent phosphorylated and nonphosphorylated HCN4 proteins.
  • N114-10_purified_HCN4_Antibody_2_112018
    IHC staining of purified anti-HCN4 antibody (clone N114/10) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R, the tissue was incubated with 5 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
  • N114-10_purified_HCN4_Antibody_3_112018
    IHC staining of purified anti-HCN4 antibody (clone N114/10) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R, the tissue was incubated with 5 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
Cat # Size Price Quantity Avail. Save
857001 25 µg £47
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857002 100 µg £94
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Description

Hyperpolarization activated cyclic nucleotide gated ion channel 4 (HCN4) is a subunit of a total 4 HCN protein family that carries inward current with the feature knows as If in the heart and Ih in the brain. These currents have been indicated in a wide array of physiological activities, including pacemaker activity of neurons, and cardiomyocytes, control of resting membrane potential, neuronal plasticity, dendrite formation and response to sour taste.  Although widely expressed in the central nervous system, HCN4 is enriched in regions regulating mood and anxiety including thalamus, amygdala, midbrain dopamine system, and indirectly prefrontal cortex. SNP genotyping indicates the association of HCN4 with mood anxiety disorders. In cardiomycates, HCN4 is required for pacemaker potential generation. Mice lacking HCN4 globally or in cadiomyocytes are embryonic lethal.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Fusion protein corresponding to amino acids 1021-1110 (cytoplasmic C-terminus) of human HCN4
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 2.5 - 10 µg per ml. For immunohistochemistry on formalin-fixed paraffin-embedded tissues, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2783467 (BioLegend Cat. No. 857001)
AB_2783468 (BioLegend Cat. No. 857002)

Antigen Details

Structure
HCN4 is a 1,186 amino acid protein with a molecular mass of 127 kD.
Distribution

Tissue distribution: Brain, heart, adrenal gland, and testis.
Cellular distribution: Membrane.

Function
HCN4 generates inward current If in the heart and Ih in the brain, and modulates intrinsic neuronal excitability and synaptic integrity.
Interaction
HCN4 interacts with other HCN subunits (HCN1-3) to form a functional channel.
Biology Area
Cell Biology, Neuroscience, Synaptic Biology
Molecular Family
Presynaptic proteins
Antigen References
  1. Kelmendi, B. et al. 2011. Neurosci Lett. 496(3): 195–199.
  2. Stieber, J. et al. 2003. Proc Natl Acad Sci U S A. 100(25):15235-40.
  3. Xu, X. et al. 2010. J Biol Chem. 285(47):37082-91.
Gene ID
330953 View all products for this Gene ID 59266 View all products for this Gene ID
UniProt
View information about HCN4 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 11/21/2018

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