- Poly9023 (See other available formats)
- Regulatory Status
- Other Names
- HA epitope tag, HA1, HA2, hemagglutinin, Hemagglutinin HA1 chain, Hemagglutinin HA2 chain, YPYDVPDYA, Hemagglutinin tag
Covance Catalog# PRB-101P
- Rabbit Polyclonal IgG
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- Product Citations
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The HA tag (hemagglutinin) is an amino acid sequence derived from the human influenza hemagglutinin surface glycoprotein, corresponding to amino acids 98-106. It is commonly used as a tag to facilitate detection, isolation, and purification of proteins. The full amino acid sequence is: YPYDVPDYA.Product Details
- Antibody Type
- Host Species
- Polyclonal antibody HA.11 was raised against the twelve amino acid sequence CYPYDVPDYASL.
- Phosphate-buffered solution + 0.03% thimerosal.
- The antibody was purified by affinity chromatography.
- 1.0 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
ChIP, ICC, Purification - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting.
The optimal working dilution should be determined for each specific assay condition.
• WB: 1:1,000*
• IP: 1:150
- Application Notes
This antibody is effective in immunoblotting (WB) and immunoprecipitation (IP) of tagged proteins.
*Our Posi-Tag Control Protein (931301) can be used as a helpful positive control for this antibody.
This antibody recognizes the same hemagglutinin flu epitope YPYDVPDYA as the monoclonal antibody, HA.11 clone 16B12. This polyclonal antibody facilitates epitope tag experiments in murine systems and is useful in experiments involving two proteins labeled with two different tags.
This product may contain other non-IgG subtypes.
(PubMed link indicates BioLegend citation)
- Barrott JJ, et al. 2011. PNAS. 108: 12752. (WB, IP)
- Pecot MY, Malhotra V. 2004. Cell. 116:99.
- Delmas C, et al. 2003. J Biol Chem. 278:12443. (IF)
- Caplan S, et al. 2001. J Cell Biol. 154:109. (IF, WB)
- Yeo C, Whitman M. 2001. Mol Cell 7:949.
- Sander TL, et al. 2000. J Biol Chem.:12857. (WB)
- Bosshart H, et al. 1994. J Cell Biol. 126:1157. (IF, IP)
- Hewawasam G, et al. 2010. Mol Cell. 40:444. (ChIP, WB) PubMed
- Hurov JB, et al. 2004. Curr Biol. 14:736. (IF)
- Shieh AW, et al. 2013. PLoS One. 8:75337. (WB, IF) PubMed
- Hackenbeck T, et al. 2009. Cell Cycle. 8:1386. (ICC)
- Satheshkumar PS, et al. 2013. J Virol. 87:10710.
- Luo R, et al. 2009. Cell Signal. 21:1169. (WB) PubMed
- Berg TJ, et al. 2010. J Biol Chem. 285:35255. (WB) PubMed
- Product Citations
AB_2734670 (BioLegend Cat. No. 902303)
AB_2565018 (BioLegend Cat. No. 902301)
AB_2565019 (BioLegend Cat. No. 902302)
- Biology Area
- Cell Biology
- Gene ID
- View information about HA.11 on UniProt.org
|Anti-HA Tag||Poly9023||WB, IP|
|Purified anti-HA.11 Epitope Tag||Poly9023||WB, IP, ChIP, ICC, Purification|
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