- W17173C (See other available formats)
- Other Names
- Glycogen Synthase Kinase, Glycogen Synthase Kinase 3 Alpha, Glycogen Synthase Kinase Beta, GSK3A, GSK3B
- Rat IgG2a, κ
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- Product Citations
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GSK-3α/β is a ubiquitously expressed serine/threonine kinase that can phosphorylate a wide range of substrates. While GSK-3 was initially shown to regulate energy metabolism through phosphorylation of glycogen synthase, it has been shown that the enzyme has a wide variety of substrates in vivo and regulates signaling pathways that control cell growth, development, apoptosis, and oxidative stress responses. Each isoform if encoded by separate alleles; the isoforms show 66% homology.Product Details
- Human, Mouse
- Antibody Type
- Host Species
- Recombinant full-length GSK-3β
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
WB - Quality tested
IP - Validated
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 µg per ml. For immunoprecipitation, the suggested use of this reagent is 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
This clone recognizes both α and β isoforms of GSK-3 by both WB and IP.
This clone failed to stain PFA-fixed HeLa cells that were permeabilized with methanol or Triton-X. We therefore do not recommend using this clone for ICC.
- GSK-3α is a 483 amino acid protein with a predicted molecular weight of 51 kD; GSK-3β is a 420 amino acid protein with a predicted molecular weight of 47 kD.
Both isoforms ubiquitously expressed
GSK-3α: cytosol; GSK-3β: nucleus
- Cell Signaling
- Antigen References
- Imazu M, et al. 1984. J. Biol. Chem. 259: 1813.
- Cross DA, et al. 1995. Nature. 378: 785.
- Kazi A, et al. 2018. Nat. Commun. 9: 5154.
- Gene ID
- 2931 View all products for this Gene ID 2932 View all products for this Gene ID
- View information about GSK-3alpha/beta on UniProt.org
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