Purified anti-GRB2 Antibody

Pricing & Availability
Clone
W17230A (See other available formats)
Other Names
Growth Factor Receptor Bound Protein 2, SH2/SH3 Adaptor Protein GRB2, ASH, Epidermal Growth Factor Receptor-Binding Protein GRB2, Abundant SRC Homology, MSTP084, Grb3-3, MST084, NCKAP2
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W17230A_PURE_GRB2_Antibody_062519
Whole cell extracts (15 µg protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) of purified anti-GRB2 antibody, clone W17230A, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular Weight marker.
  • W17230A_PURE_GRB2_Antibody_062519
    Whole cell extracts (15 µg protein) from the indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL (1:500 dilution) of purified anti-GRB2 antibody, clone W17230A, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular Weight marker.
Cat # Size Price Quantity Avail. Save
627001 25 µg £57
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627002 100 µg £144
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Description

GRB2 is a receptor tyrosine kinase (RTK) adaptor protein that transduces signaling cascades from activated receptors. Upon receptor stimulation, GRB2 binds phosphorylated RTK tyrosine residues via a central SH2 domain; flanking GRB2 SH3 domains facilitate the recruitment of downstream effectors to the activated RTK.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Synthetic peptide corresponding to amino acid residues 199-214 of human GRB2.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone has not been tested for ICC or IP.

Antigen Details

Structure
GRB2 is a 217 amino acid protein with a predicted molecular weight of 25 kD.
Distribution

Plasma membrane-associated/ubiquitously expressed

Function
Adaptor protein in RTK signaling pathways
Biology Area
Cell Biology, Signal Transduction
Molecular Family
Adaptor Proteins, Phospho-Proteins
Antigen References
  1. Lowenstein EJ, et al. 1992. Cell. 70:431-42.
  2. Matuoka K, et al. 1992. Proc Natl Acad Sci U S A. 89:9015-9.
  3. Skolnik EY, et al. 1993. Science. 260:1953-5.
  4. Pendergast AM, et al. 1993. Cell. 75:175-85.
Gene ID
2885 View all products for this Gene ID
UniProt
View information about GRB2 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 06/25/2019

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