- W18177A (See other available formats)
- Other Names
- Glutamate Dehydrogenase 1, EC 126.96.36.199, GLUD, Epididymis Tissue Sperm Binding Protein Li 18mP, Epididymis Secretory Sperm Binding Protein, GDH 1
- Rat IgG2b, κ
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- Product Citations
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Glutamate dehydrogenase (GLUD) is a mitochondrial enzyme involved in the reversible oxidative deamination of glutamate to ammonia and α-ketoglutarate (α-KG), which is a key intermediate in the tricarboxylic acid (TCA) cycle. α-KG is utilized to produce ATP, which in turn increases the ATP/ADP ratio and induces insulin secretion. GLUD mutations are associated with hyperinsulinism-hyperammonemia (HI/HA) syndrome. Humans have two isoenzymes, GLUD1 and GLUD2, each encoded by distinct genes. GLUD1 is widely expressed while GLUD2 is expressed in neural and testicular tissues.Product Details
- Human, Mouse
- Antibody Type
- Host Species
- Partial recombinant human GLUD1 protein
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography.
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
WB - Quality tested
ICC - Validated
- Recommended Usage
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
This clone recognizes both human and mouse GLUD1.
Methanol permeabilization is recommended for ICC testing. Triton X-100 permeabilization showed weak signal.
- GLUD1 is a 558 amino acid protein with a predicted molecular weight of 61 kD.
Mitochondrial matrix/ubiquitously expressed
- Biology Area
- Cell Biology, Mitochondrial Function
- Molecular Family
- Enzymes and Regulators
- Antigen References
- Stanley CA, et al.1998. N Engl J Med. 338:1352-7
- Plaitakis A, et al. 2000. J Neurochem. 75:1862
- Yang C, et al. 2009. Cancer Res. 69:7986
- Jin L, et al. 2015. Cancer Cell. 27:257
- Gene ID
- 2746 View all products for this Gene ID
- View information about GLUD1 on UniProt.org
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