Purified anti-GLUD1 Antibody

Pricing & Availability
Clone
W18177A (See other available formats)
Other Names
Glutamate Dehydrogenase 1, EC 1.4.1.3, GLUD, Epididymis Tissue Sperm Binding Protein Li 18mP, Epididymis Secretory Sperm Binding Protein, GDH 1
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
W18177A_PURE_GLUD1_Antibody_1_111219_updated.png
Whole cell extracts (15 µg protein) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel, transferred to PVDF and probed with 0.25 µg/mL (1:2000 dilution) of purified anti-GLUD1 antibody (clone W18177A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG Antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular weight marker.
  • W18177A_PURE_GLUD1_Antibody_1_111219_updated.png
    Whole cell extracts (15 µg protein) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel, transferred to PVDF and probed with 0.25 µg/mL (1:2000 dilution) of purified anti-GLUD1 antibody (clone W18177A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG Antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular weight marker.
  • W18177A_PURE_GLUD1_Antibody_2_103019
    A549 cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with methanol for 10 minutes, and blocked with 5% FBS for 60 minutes. Cells were then intracellularly stained with 1.0 µg/mL (1:500 dilution) of purified anti-GLUD1 antibody (clone W18177A, panel B) overnight at 4°C, followed by incubation with Alexa Fluor® 594 Goat anti-rat IgG Antibody (Cat. No. 405422) at 2.0 µg/mL and Alexa Fluor® 647 anti-Cytochrome c Antibody (Cat. No. 612310) at 2.0 µg/mL. Cytochrome C uses green pseudo-color for visualization (panel A). Nuclei were counterstained with DAPI, and the image was captured with a 60X objective.
Cat # Size Price Quantity Avail. Save
933601 25 µg £58
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933602 100 µg £147
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Description

Glutamate dehydrogenase (GLUD) is a mitochondrial enzyme involved in the reversible oxidative deamination of glutamate to ammonia and α-ketoglutarate (α-KG), which is a key intermediate in the tricarboxylic acid (TCA) cycle. α-KG is utilized to produce ATP, which in turn increases the ATP/ADP ratio and induces insulin secretion. GLUD mutations are associated with hyperinsulinism-hyperammonemia (HI/HA) syndrome. Humans have two isoenzymes, GLUD1 and GLUD2, each encoded by distinct genes. GLUD1 is widely expressed while GLUD2 is expressed in neural and testicular tissues.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partial recombinant human GLUD1 protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
ICC - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone recognizes both human and mouse GLUD1.

Methanol permeabilization is recommended for ICC testing. Triton X-100 permeabilization showed weak signal.

Antigen Details

Structure
GLUD1 is a 558 amino acid protein with a predicted molecular weight of 61 kD.
Distribution

Mitochondrial matrix/ubiquitously expressed

Function
Glutaminolysis
Biology Area
Cell Biology, Mitochondrial Function
Molecular Family
Enzymes and Regulators
Antigen References
  1. Stanley CA, et al.1998. N Engl J Med. 338:1352-7
  2. Plaitakis A, et al. 2000. J Neurochem. 75:1862
  3. Yang C, et al. 2009. Cancer Res. 69:7986
  4. Jin L, et al. 2015. Cancer Cell. 27:257
Gene ID
2746 View all products for this Gene ID
UniProt
View information about GLUD1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 10/30/2019

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