Purified anti-GGT1 Antibody

Pricing & Availability
Clone
BL28543 (See other available formats)
Other Names
GGT1, Gamma-glutamyltransferase 1
Isotype
Mouse IgG1, κ
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Product Citations
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BL28543_Pure_GGT1_Antibody_1_111218
Western blot of purified anti-GGT1 antibody (clone BL28543). Lane 1: Molecular weight marker; Lane 2: 20 µg of HepG2 Cell lysate; Lane 3: 20 µg of human liver lysate. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • BL28543_Pure_GGT1_Antibody_1_111218
    Western blot of purified anti-GGT1 antibody (clone BL28543). Lane 1: Molecular weight marker; Lane 2: 20 µg of HepG2 Cell lysate; Lane 3: 20 µg of human liver lysate. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • BL28543_Pure_GGT1_Antibody_2_111218
    IHC staining of purified anti-GGT1 antibody (clone BL28543) on formalin-fixed paraffin-embedded human kidney tissue. Following antigen retrieval using Sodium Citrate H.I.E.R, the tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
Cat # Size Price Quantity Avail. Save
854301 25 µg £60
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Description

GGT1 is a transferase that plays a key role in the gamma-glutamyl cycle. GGT1 is abundantly expressed in liver and kidney. Its involvement in glutathione metabolism makes it an important regulator of the oxidative stress in the central nervous system, as well as in other tissues. GGT1 is also a known marker for apoptosis. Importantly, elevated levels of GGT1 correlates with breast cancer tumor progression and resistance to chemotherapy, suggesting GGT1 as a candidate for cancer prognosis marker.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
0.01 M PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
Store at 4°C for up to 3 months. For longer term storage aliquot into small volumes and store at -20°C
Application

WB - Quality tested
IHC-P - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 5.0 µg per ml. For immunohistochemistry on formalin-fixed paraffin-embedded tissue, a concentration range of 1.0 - 5.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2783438 (BioLegend Cat. No. 854301)

Antigen Details

Structure
GGT1 is a 569 amino acid protein with a molecular mass of ~ 61 kD.
Distribution

Tissue Distribution: Kidney, liver, pancreas, stomach, intestine, placenta, and lung.
Cellular distribution: Mainly on plasma membrane or extracellular

Function
Cleaves the gamma-glutamyl bond of extracellular glutathione, maintains intracellular GSH level.
Interaction
Heterodimer forms by interactions between light and heavy chains.
Biology Area
Cell Biology, Neuroinflammation, Neuroscience, Synaptic Biology
Molecular Family
Postsynaptic proteins, Presynaptic proteins
Antigen References
  1. Priolo C, et al. 2018. Proc Natl Acad Sci U S A. 115(27):E6274
  2. Darin N, et al. 2018. Eur J Hum Genet. 26(6):808
  3. Yoshikawa C. 1995. Nihon Rinsho. 53(5): 1162
Gene ID
2678 View all products for this Gene ID
UniProt
View information about GGT1 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 11/14/2018

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