Purified anti-Gephyrin Antibody

Pricing & Availability
Clone
O93A6 (See other available formats)
Other Names
GPHN, Gephyrin
Isotype
Mouse IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
publications
O93A6_PURE_Gephyrin_Antibody_1_100918
Western blot of purified anti-Gephyrin antibody (clone O93A6). Lane 1: Molecular weight marker; Lane 2: 10 µg of human brain lysate; Lane 3: 10 µg of mouse brain lysate; Lane 4: 10 µg of rat brain lysate. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • O93A6_PURE_Gephyrin_Antibody_1_100918
    Western blot of purified anti-Gephyrin antibody (clone O93A6). Lane 1: Molecular weight marker; Lane 2: 10 µg of human brain lysate; Lane 3: 10 µg of mouse brain lysate; Lane 4: 10 µg of rat brain lysate. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • O93A6_PURE_Gephyrin_Antibody_2_100918
    IHC staining of purified anti-Gephyrin antibody (clone O93A6) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928502), the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm.
  • O93A6_PURE_Gephyrin_Antibody_3_100918
    IHC staining of purified anti-Gephyrin antibody (clone O93A6) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928502), the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm.
  • O93A6_PURE_Gephyrin_Antibody_4_100918
    IHC staining of purified anti-Gephyrin antibody (clone O93A6) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928502), the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm.
Cat # Size Price Quantity Avail. Save
856101 25 µg £60
Check Availability


Need larger quantities of this item?
Request Bulk Quote
856102 100 µg £158
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

Gephyrin (GPHN) is a scaffold protein that anchors glycine and GABA receptors to the tubulin cytoskeleton in the postsynaptic terminal of inhibitory synapses. Gephyrin's function as a scaffolding protein is regulated at transcriptional and translational levels, which impact the formation and plasticity of GABAergic synapses, as well as the overall strength of GABAergic transmission. Low levels of gephyrin in temporal lobe correspond to reduced inhibitory signals and correlate with temporal lobe epilepsy.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human recombinant gephyrin expressed in human HEK293T cells.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 5.0 µg per ml. For immunohistochemistry, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2783450 (BioLegend Cat. No. 856101)
AB_2783451 (BioLegend Cat. No. 856102)

Antigen Details

Structure
Gephyrin is a 736 amino acid protein with an apparent molecular mass of ~ 93 kD.
Distribution

Tissue Distribution: Nervous system, endocrine system, and gastrointestinal tract.
Cellular Distribution: Plasma membrane, cytoskeleton, and cytosol.

Function
Gephyrin is a scaffolding protein that anchors inhibitory neurotransmitter receptors to the postsynaptic cytoskeleton.
Interaction
Homodimer and homo-oligomer, interacts with GLRB, GABARAP, GABRA3, GLRB.
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers, Synaptic Biology
Molecular Family
Postsynaptic proteins
Antigen References
  1. Alvarez FJ. 2017. Brain Res. Bull. 129:50.
  2. Tyagarajan SK, Fritschy JM. 2014. Nat. Rev. Neurosci. 15:141-56.
  3. Zacchi P, et al. 2014. Front Cell. Neurosci. 8:103.
Gene ID
10243 View all products for this Gene ID 268566 View all products for this Gene ID 64845 View all products for this Gene ID
UniProt
View information about Gephyrin on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 10/10/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 9727 Pacific Heights Blvd, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Login / Register
Forgot your password? Reset password?
Create an Account