Purified anti-Gemin 3 Antibody

Pricing & Availability
Clone
12H12 (See other available formats)
Other Names
Gemin3
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
12H12_PURE_Gemin3_Antibody_051019
Western blot of purified anti-Gemin 3 antibody (clone 12H12). Lane 1: Molecular weight marker; Lane 2: 20µg of HeLa cell lysate; Lane 3: 10µg of HeLa cell lysate; Lane 4: 5µg of HeLa cell lysate. The blot was incubated with 2 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • 12H12_PURE_Gemin3_Antibody_051019
    Western blot of purified anti-Gemin 3 antibody (clone 12H12). Lane 1: Molecular weight marker; Lane 2: 20µg of HeLa cell lysate; Lane 3: 10µg of HeLa cell lysate; Lane 4: 5µg of HeLa cell lysate. The blot was incubated with 2 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
Cat # Size Price Quantity Avail. Save
865501 25 µg £60
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865502 100 µg £158
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Description

Gemin 3, also known as DEAD-box helicase 20 (DDX20), is a putative RNA helicase that contains a conserved ASP-GLU-ALA-ASP (DEAD) motif. Gemin 3 is involved in a variety of cellular processes including initiation of protein translation, ribosome assembly, and alteration of RNA secondary structure. Gemin 3 is one of the core components of the survival of motor neurons (SMN) macromolecule protein complex, which plays an important role in the assembly and re-structuring of small nucleoproteins and transcriptosomes. Gemin 3 may also be involved in cancer as it acts as a tumor suppressor in hepatocellular carcinoma, and as a tumor promoter in breast cancer and prostate cancer2.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Antigen Details

Structure
Gemin 3 is an 824 amino acid protein with an apparent molecular mass of ~ 92 kD.
Distribution

Tissue Distribution: Testis and ovary
Cellular Distribution: Cytosol, nucleus, and cytoskeleton

Function
Gemin 3 is a component of SMN macromolecule protein complex.
Interaction
SMN, GEMIN4
Cell Type
Astrocytes, GABAergic Neurons, Glutamatergic Neurons, Mature Neurons, Microglia, Neural Stem Cells, Neurons, Oligodendrocytes
Biology Area
Cancer Biomarkers, Neurodegeneration, Neuroscience, Synaptic Biology, Transcription Factors
Antigen References
  1. Seng CO, et al. 2015. Hum. Mol. Genet. 24(8):2138.
  2. Chen W, et al. 2016. Int. J. Mol. Med. 37(6):1551.
Gene ID
11218 View all products for this Gene ID
UniProt
View information about Gemin 3 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 05/10/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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