Purified anti-Gelsolin Antibody

Pricing & Availability
Clone
CPTC-Gelsolin-2 (See other available formats)
Other Names
GSN, AGEL, Actin-depolymerizing factor, ADF, Brevin, Epididymis Secretory Sperm Binding Protein, Amyloidosis, Finnish Type
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
CPTC-Gelsolin-2_PURE_Gelsolin_Antibody_1_062719.png
Western blot of purified anti-Gelsolin antibody (clone CPTC-Gelsolin-2). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • CPTC-Gelsolin-2_PURE_Gelsolin_Antibody_1_062719.png
    Western blot of purified anti-Gelsolin antibody (clone CPTC-Gelsolin-2). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • CPTC-Gelsolin-2_PURE_Gelsolin_Antibody_2_062719.png
    Western blot of purified anti-Gelsolin antibody (clone CPTC-Gelsolin-2). Lane 1: Molecular weight marker; Lane 2: 20 µg of Drosophila S2 cell lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • CPTC-Gelsolin-2_PURE_Gelsolin_Antibody_3_062719.png
    IHC staining of purified anti-Gelsolin antibody (clone CPTC-Gelsolin-2) on formalin-fixed paraffin-embedded glioblastoma brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/mL of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • CPTC-Gelsolin-2_PURE_Gelsolin_Antibody_4_062719.png
    IHC staining of purified anti-Gelsolin antibody (clone CPTC-Gelsolin-2) on formalin-fixed paraffin-embedded normal human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/mL of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
Cat # Size Price Quantity Avail. Save
866501 25 µg £60
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866502 100 µg £158
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Description

After severing, gelsolin remains bound to the barbed end of the actin filament, preventing the reannealing of severed filaments. Gelsolin is crucial for the maintenance of actin homeostasis. Cells from gelsolin null mice exhibit a variety of defects, such as pronounced stress fibers formation, defective chemotaxis, and wound healing. Dysfunction of Gelsolin has been indicated in multiple diseases include cancer, infection, inflammation, cardiac injury, pulmonary disease, and neurodegeneration. Gelsolin is proteolytically cleaved and rendered inactive in Alzheimer’s disease (AD).. Overexpression of gelsolin significantly reduces amyloid beta load in brains of AD transgenic mice.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Drosophila
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant full-length gelsolin protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1 - 5 µg per ml. For formalin-fixed paraffin-embedded immunohistochemical staining, a concentration range of 1 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Antigen Details

Structure
Human gelsolin is a 782 amino acid protein with a molecular mass of 86 kD.
Distribution

Tissue distribution: Central nervous system, phagocytic cells, platelets, fibroblasts, non-muscle cells, smooth and skeletal muscle cells.
Cellular distribution: Cytoskeleton and extracellular

Function
Gelsolin is a calcium-binding, actin-modulating protein. It can promote the assembly of actin monomers into filaments as well as sever actin filaments.
Interaction
Interacts with actin and fibronectin. Identified in a complex composed of ACTA1, COBL, GSN AND TMSB4X.
Cell Type
Astrocytes, Microglia, Neurons, Oligodendrocytes
Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Cell Structure, Neurodegeneration, Neuroscience
Molecular Family
Cytoskeletal Proteins
Antigen References
  1. Ji L, et al. 2015. J Alzheimers Dis. 44(1):13-25
  2. Asch HL, et al. 1996. Cancer Res. 56(21):4841-5.
  3. Sun HQ, et al. 1999. J Biol Chem. 274(47):33179-82.
  4. Ji L, et al. 2009. J Alzheimers Dis. 18(1):105-11.
Gene ID
2934 View all products for this Gene ID
UniProt
View information about Gelsolin on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 06/27/2019

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