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Purified anti-Galectin-1 Antibody

Pricing & Availability
Clone
A18049E (See other available formats)
Regulatory Status
RUO
Other Names
Galectin 1, LGALS1, Gal-1
Isotype
Rat IgG2a, κ
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Product Citations
publications
A18049E_PURE_Galectin-1_Antibody_082119
Western blot of purified anti-Galectin-1 antibody (clone A18049E). Lane 1: Molecular weight marker; Lane 2: 10 µg of human brain lysate; Lane 3: 10 µg of HeLa cell lysate. The blot was incubated with 2 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • A18049E_PURE_Galectin-1_Antibody_082119
    Western blot of purified anti-Galectin-1 antibody (clone A18049E). Lane 1: Molecular weight marker; Lane 2: 10 µg of human brain lysate; Lane 3: 10 µg of HeLa cell lysate. The blot was incubated with 2 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
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867301 25 µg £73
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867302 100 µg £189
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Description

Galectin-1 is a secreted carbohydrate-binding protein that interacts with β-galactoside to modulate cell-cell or cell-matrix interactions. Galectin-1 is expressed in all gliomas and its expression level correlates directly with the grade of astrocytic tumor.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Full-length recombinant human Galectin-1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 5.0 - 10 µg per ml. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2814612 (BioLegend Cat. No. 867301)
AB_2814613 (BioLegend Cat. No. 867302)

Antigen Details

Structure
Galectin 1 is a 135 amino acid protein with predicted and observed molecular mass of ~15 kD.
Distribution

Tissue Distribution: Smooth muscle, placenta, endometrium, adipose tissue, and soft tissue

Cellular Distribution: Endoplasmic reticulum, cytosol, nucleus, plasma membrane, and extracellular

Function
Galectin 1 binds to β-galactoside and is involved in regulating apoptosis, cell proliferation, and differentiation.
Interaction
Galectin 1 forms homodimers and binds to LGALS3BP, GEMIN4, HRAS, Laminin, SUSD2.
Cell Type
Microglia
Biology Area
Cancer Biomarkers, Neurodegeneration, Neuroinflammation, Neuroscience, Neuroscience Cell Markers
Antigen References
  1. Gajiwala S, et al. 2018. J. Gastrointest. Oncol. 9:1014-26. 
  2. Seropian IM, et al. 2018. Mediators Inflamm. 2018:8696543.
  3. Brinchmann MF, et al. 2018. Mediators Inflamm. 2018:9186940.
Gene ID
2956 View all products for this Gene ID
UniProt
View information about Galectin-1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 08/21/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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