Purified anti-FXR1 Antibody

Pricing & Availability
Clone
6BG10 (See other available formats)
Other Names
FMR1 Autosomal Homolog 1, Fragile X Mental Retardation Syndrome-Related Protein 1
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
N308-48_HRP_NMDAR1_Antibody_1_061819
Western blot of purified anti-FXR1 antibody (clone 6BG10). Lane 1: Molecular weight marker; Lane 2: 40 µg of human brain membrane lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • N308-48_HRP_NMDAR1_Antibody_1_061819
    Western blot of purified anti-FXR1 antibody (clone 6BG10). Lane 1: Molecular weight marker; Lane 2: 40 µg of human brain membrane lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • N308-48_HRP_NMDAR1_Antibody_2_061819
    IHC staining of purified anti-FXR1 antibody (clone 6BG10) on formalin-fixed paraffin-embedded human cerebellum tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928502), the tissue was incubated with 2 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
Cat # Size Price Quantity Avail. Save
867001 25 µg £60
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867002 100 µg £158
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Description

FXR1 is an RNA binding protein that interacts with polyribosomes, specifically with the 60S ribosome subunit. In the adult brain, FXR1 is mainly localized to the cytoplasm and the proximal dendrites of neurons. In the fetal brain, FXR1 is expressed in the nucleus of neurons. Clinically, FXR1 mutations have been identified as a major genetic contributing factor to fragile X mental retardation syndrome. Recent studies also suggest that FXR family of proteins integrate dopamine signaling and glutamatergic neurotransmission.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human FXR1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P - Validated
ICC - Reported in the literature

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 2 - 10 µg per ml. For immunohistochemistry, a concentration range of 1 - 5 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1.     Xu XL, et al. 2011. J. Neurosci. 31(39):13705-9. (WB)
  2.     Battle DJ, et al. 2007. J. Biol. Chem. 282(38):27953-9. (ICC)

Antigen Details

Structure
FXR1 is a 621 amino acid protein with predicted molecular mass of ~ 70 kD and observed molecular mass of ~ 75kD.
Distribution

Tissue Distribution: Ubiquitously expressed
Cellular Distribution: Cytosol and nucleus

Function
FXR1 is an RNA binding protein involved in neurodevelopmental diseases such as autism, schizophrenia, and fragile X mental retardation.
Interaction
Polyribosome, FXR2, FMR1, CYFIP2
Cell Type
Dopaminergic Neurons, GABAergic Neurons, Neurons, Oligodendrocytes
Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Signal Transduction, Synaptic Biology, Transcription Factors
Molecular Family
Postsynaptic proteins, Presynaptic proteins
Antigen References
  1.     Khlghatyan J, Beaulieu JM. 2018. Front Synaptic. Neurosci. 10:22.
  2.     Bardoni B, et al. 2001. Brain Res. Bull. 56:375-82.
  3.     Inoue SB, et al. 2000. J. Med. Invest. 47(3-4):101.
Gene ID
8087 View all products for this Gene ID
UniProt
View information about FXR1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 06/18/2019

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